Parvalbumin and Ubiquitin as Potential Biomarkers of Mercury Contamination of Amazonian Brazilian Fish
Nenhuma Miniatura disponível
Data
2020-10-01
Autores
Vieira, José Cavalcante Souza [UNESP]
de Oliveira, Grasieli [UNESP]
Braga, Camila Pereira
da Silva Fernandes, Mileni
de Moraes, Paula Martin
Buzalaf, Marília Afonso Rabelo
de Oliveira, Lincoln Carlos Silva
de Magalhães Padilha, Pedro [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Recent studies have demonstrated the association of mercury (Hg) with some fish proteins, milk, and hair from individuals exposed to the element in the Amazon. However, few studies involve identifying biomarkers of mercury exposure. Therefore, the present study aimed to identify potential biomarkers of Hg exposure in fish. For this, the muscular tissues of two species of fish (Prochilodus lineatus and Mylossoma duriventre) that feed the Amazonian human population were analyzed. Through the analyses obtained by graphite furnace atomic absorption spectrometry (GFAAS), it was possible to identify four protein SPOTS where mercury was present. These SPOTS, identified by mass spectrometry (ESI-MS/MS), included parvalbumin and ubiquitin-40S ribosomal protein S27a, and these being metalloproteins with biomarker characteristics. In addition, the results show the intense Hg/protein ratio observed in the two proteins, which makes metalloproteins strong candidates for biomarkers of mercury exposure. [Figure not available: see fulltext.].
Descrição
Palavras-chave
Biomarkers, Fish, Mercury in the Amazon, Mercury-bound proteins, Metalloproteins, Proteomics
Como citar
Biological Trace Element Research, v. 197, n. 2, p. 667-675, 2020.