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dc.contributor.authorRocha, Lucas Fernandes [UNESP]
dc.contributor.authorPaula, Natália Ribeiro
dc.contributor.authorNazareno, Alison Gonçalves
dc.contributor.authorDe Carvalho, Dulcinéia
dc.date.accessioned2020-12-12T01:30:30Z
dc.date.available2020-12-12T01:30:30Z
dc.date.issued2020-07-07
dc.identifierhttp://dx.doi.org/10.1186/s40659-020-00298-z
dc.identifier.citationBiological Research, v. 53, n. 1, 2020.
dc.identifier.issn0717-6287
dc.identifier.issn0716-9760
dc.identifier.urihttp://hdl.handle.net/11449/199089
dc.description.abstractBackground: We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. Results: Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed (H O ) and expected (H E ) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively. Conclusions: These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy.en
dc.language.isoeng
dc.relation.ispartofBiological Research
dc.sourceScopus
dc.subjectEremanthus erythropappus
dc.subjectEremanthus glomerulatus
dc.subjectEremanthus incanus
dc.subjectGenetic diversity
dc.subjectSimple sequence repeats
dc.titleDevelopment and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related speciesen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Lavras (UFLA)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.description.affiliationDepartment of Forest Science São Paulo State University UNESP
dc.description.affiliationDepartment of Forest Sciences Laboratory of Tree Genetic Conservation Federal University of Lavras UFLA
dc.description.affiliationDepartment of Biology Federal University of Minas Gerais UFMG
dc.description.affiliationUnespDepartment of Forest Science São Paulo State University UNESP
dc.identifier.doi10.1186/s40659-020-00298-z
dc.identifier.scopus2-s2.0-85087738795
unesp.author.orcid0000-0002-7094-7430[1]
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