Avaliação de compostos químicos e bactérias presentes na superfície dos ovos e nos ovários na regulação de oviposição de duas espécies de moscas-varejeiras de importância forense
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Data
2021-01-27
Autores
Lima, Thiago de [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A escolha do local de oviposição em moscas-varejeiras é de suma importância para um desenvolvimento adequado das larvas e futuros adultos, essa escolha geralmente é feita analisando pistas químicas, voláteis ou não, e micro-organismos presentes no ambiente. E independentemente do substrato, as moscas-varejeiras sempre optam por colocar seus ovos agregados à ovos coespecíficos, pois isso garante vantagens à prole. Porém, o motivo pelo qual as fêmeas realizam esse comportamento ainda não é completamente compreendido. Uma das hipóteses é que os ovos e os ovários contêm semioquímicos e bactérias que auxiliam na regulação desse comportamento. Os objetivos desta pesquisa foram descobrir se extratos de ovos em diferentes estágios de desenvolvimento e ovários coespecíficos provocam atração em fêmeas de C. megacephala e C. albiceps e, em caso positivo, traçar o perfil químico dessas estruturas e, em seguida, descrever a comunidade bacteriana da superfície desses ovos e ovários para averiguar a presença de possíveis compostos e micro-organismos que possam afetar o comportamento reprodutivo de alguma maneira, especialmente o de oviposição. Os resultados mostraram que alguns extratos, principalmente em éter, provocam atração em fêmeas e que tanto os compostos químicos encontrados, uma mistura complexa de hidrocarbonetos e ácidos graxos, como as bactérias podem ter um papel na regulação de oviposição, desde a atração ao local até a agregação de adultos e ovos.
The choice of oviposition site in blowflies is extremely important for an appropriate development of the larvae and future adults, this choice is usually made by analyzing chemical clues, volatile or not, and microorganisms present in the environment. Regardless the substrate, blowflies always choose to lay their eggs attached to co-specific eggs, since this behaviour guarantees advantages to their offspring. However, the reason why females carry out this behavior is not yet fully understood. One hypothesis is that eggs and ovaries contain semiochemicals and bacteria that help regulate this behavior. The objectives of this research were to find out if egg extracts at different stages of development and cospecific ovaries produce attraction in females of C. megacephala and C. albiceps and, if so, to describe the chemical profile of these structures and then describe the bacterial community on the surface of these eggs and ovaries to verify the presence of possible compounds and microorganisms that may affect reproductive behavior in some way, especially oviposition. The results showed that some extracts, mainly in ether, produce attraction in females and that the chemical compounds found, a complex mixture of hydrocarbons and fatty acids, as well as bacteria can play a role in the oviposition regulation, from attraction to the site to the aggregation of adults and eggs.
The choice of oviposition site in blowflies is extremely important for an appropriate development of the larvae and future adults, this choice is usually made by analyzing chemical clues, volatile or not, and microorganisms present in the environment. Regardless the substrate, blowflies always choose to lay their eggs attached to co-specific eggs, since this behaviour guarantees advantages to their offspring. However, the reason why females carry out this behavior is not yet fully understood. One hypothesis is that eggs and ovaries contain semiochemicals and bacteria that help regulate this behavior. The objectives of this research were to find out if egg extracts at different stages of development and cospecific ovaries produce attraction in females of C. megacephala and C. albiceps and, if so, to describe the chemical profile of these structures and then describe the bacterial community on the surface of these eggs and ovaries to verify the presence of possible compounds and microorganisms that may affect reproductive behavior in some way, especially oviposition. The results showed that some extracts, mainly in ether, produce attraction in females and that the chemical compounds found, a complex mixture of hydrocarbons and fatty acids, as well as bacteria can play a role in the oviposition regulation, from attraction to the site to the aggregation of adults and eggs.
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Entomologia forense, Ecologia química, Micro-organismos, Comportamento, Forensic entomology, Chemical ecology, Microorganisms, Behavior, Calliphoridae, Semioquímicos, Bactérias, Comportamento de oviposição, Semiochemical, Bacteria, Oviposition behavior