Caracterização epidemiológica e imunoistoquímica do linfoma multicêntrico indolente de zona t em cães
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Data
2020-12-21
Autores
Lima, Guilherme Malvezi Caetano de
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Universidade Estadual Paulista (Unesp)
Resumo
O presente projeto de pesquisa teve como objetivo principal a caracterização epidemiológica e imunoistoquímica, com a avaliação da viabilidade de transposição dos marcadores atualmente utilizados em citometria de fluxo para a técnica de imunoistoquímica, de linfomas indolentes de zona T de cães, em amostras incluídas em parafina. Para tal, foram utilizadas 39 amostras de linfoma indolente de zona T. Os dados epidemiológicos foram compilados dos prontuários dos cães acometidos e catalogados em uma planilha eletrônica para análise e as respectivas amostras de tecido foram submetidas à imunoistoquímica, empregando-se os anticorpos anti-CD3, CD45, CD79a e Ki67. Nos resultados da avaliação epidemiológica dos animais com linfomas de zona T, a raça mais acometida foi o Golden Retriever (44%), seguido pelo Shih-tzu (13%), 18 animais eram machos e 21 eram fêmeas e a média de idade dos animais acometidos foi de 8 anos e 10 meses. Através de formulários enviados via Google Forms® aos veterinários responsáveis por cada caso, foi possível obter o follow up de 23 animais, sendo que a alteração clínica mais relatada foi a linfadenomegalia generalizada (n=12;52%). Sete (30%) animais apresentavam linfocitose no momento do diagnóstico. A marcação imunoistoquímica do CD45 foi negativa em todas as amostras, tal qual nos exames de citometria de fluxo. O índice de proliferação celular Ki67 nos linfomas de zona T variou de 5-40%, com uma média de 12,7% (± 7,5). A sobrevida global foi de 256 dias (± 179) e a sobrevida específica de 334 dias (± 226), embora 14/23 animais estivessem vivos até término do estudo com acompanhamento de até 565 dias. O perfil epidemiológico do linfoma de zona T no estudo foi semelhante ao encontrado em trabalhos americanos com alta prevalência em cães da raça Golden Retriever. A perda de marcação para CD45 e baixo valor de Ki67 pode ser utilizada na rotina na diferenciação entre linfomas de zona T e linfomas de alto grau de imunofenótipo T, uma vez que são doenças com prognósticos e tratamentos distintos.
ABSTRACT – The present research project aims for the epidemiological characterization and evaluation of the viability of transposing currently used markers in flow cytometry to a immunohistochemical technique for multicentric indolent T-zone lymphoma in dogs, in samples immersed in paraffin. Therefore, there were 39 samples of indolent T-zone lymphoma. The epidemiological data were compiled from the affected dogs’ records and catalogued in an electronic spreadsheet to be analyzed and the corresponding tissue samples went through the immunohistochemical exam applying antibodies anti-CD3, CD45, CD79a e Ki67. According to the results of the epidemiological evaluation, Golden Retriever was the most affected breed (44%), followed by Shih-tzu (13%); regarding the gender, 18 animals were male and 21 female, the average age of the affected animals was 8 years and 10 months. Through forms sent via Google Forms® to the responsible veterinarians for each case, it was possible to obtain a follow-up of 23 animals in which the most reported clinical alteration was generalized lymph adenomegaly (n = 12/52%). Seven animals (30%) presented lymphocytosis at diagnosis. The immunohistochemical mark of CD45 was negative in all samples, as well as in the flow cytometry. The Ki67 cell proliferation rate in T-zone lymphomas varied 5-40%, with an average of 12,7% (± 7,5). The overall survival was 256 days (± 179) and the specific survival was 334 days (± 226), although 14/23 animals were alive until the end of the research with a follow-up of 556 days. The epidemiological profile of indolent T-zone lymphoma in the study was similar to the ones found in American studies with high prevalence in Golden Retriever dogs. The mark loss to CD45 and low value of Ki67 can be used in the routine of differentiation between T-zone lymphomas and high-grade lymphomas of immunophenotype T since they are diseases with different prognoses and distinct treatments.
ABSTRACT – The present research project aims for the epidemiological characterization and evaluation of the viability of transposing currently used markers in flow cytometry to a immunohistochemical technique for multicentric indolent T-zone lymphoma in dogs, in samples immersed in paraffin. Therefore, there were 39 samples of indolent T-zone lymphoma. The epidemiological data were compiled from the affected dogs’ records and catalogued in an electronic spreadsheet to be analyzed and the corresponding tissue samples went through the immunohistochemical exam applying antibodies anti-CD3, CD45, CD79a e Ki67. According to the results of the epidemiological evaluation, Golden Retriever was the most affected breed (44%), followed by Shih-tzu (13%); regarding the gender, 18 animals were male and 21 female, the average age of the affected animals was 8 years and 10 months. Through forms sent via Google Forms® to the responsible veterinarians for each case, it was possible to obtain a follow-up of 23 animals in which the most reported clinical alteration was generalized lymph adenomegaly (n = 12/52%). Seven animals (30%) presented lymphocytosis at diagnosis. The immunohistochemical mark of CD45 was negative in all samples, as well as in the flow cytometry. The Ki67 cell proliferation rate in T-zone lymphomas varied 5-40%, with an average of 12,7% (± 7,5). The overall survival was 256 days (± 179) and the specific survival was 334 days (± 226), although 14/23 animals were alive until the end of the research with a follow-up of 556 days. The epidemiological profile of indolent T-zone lymphoma in the study was similar to the ones found in American studies with high prevalence in Golden Retriever dogs. The mark loss to CD45 and low value of Ki67 can be used in the routine of differentiation between T-zone lymphomas and high-grade lymphomas of immunophenotype T since they are diseases with different prognoses and distinct treatments.
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Palavras-chave
Cão, Linfoma de zona T, Imunoistoquímica, Golden retriever, T zone lymphoma, Dog, Immunohistochemistry