Publicação: Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers
Carregando...
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Tipo
Artigo
Direito de acesso
Resumo
The objective was to investigate effects of progesterone (P-4) dose on abundance of luteinizing hormone receptor (LHCGR), aromatase (CYP19A1), 3 beta-hydroxysteroid dehydrogenase (HSD3B1), and other steroidogenic mRNA transcripts in granulosa cells from dominant follicles. Nellore heifers were assigned to one of six groups: new, first-use controlled internal drug release device (CIDR1) inserted for 5 days (Large-P-4-dose-D5; n = 7) or 6 days (Large-P-4-dose-D6; n = 8), prostaglandin (PG)F-2 alpha administered on DO and 1 previously-used CIDR (CIDR3) inserted for 5 days (Small-P-4-dose-D5; n = 8) or 6 days (Small-P-4-dose-D6; n = 8), CIDR1 inserted on DO and removed plus PGF(2 alpha) on D5 (Large-P-4-dose-pmestrus (PE); n = 7), and CIDR3 and PGF(2 alpha) on DO and 1, CIDR3 removed plus PGF(2 alpha) on D5 (Small-P-4-dose-PE; n = 7). Duration of P-4 treatment (D5 compared to D6) affected abundances of CYP19A1 mRNA transcripts, with there being greater abundances on D6 than D5 (P <= 0.05). Heifers treated with the large dose of P-4 had a smaller dominant follicle, less serum and intra-follicular estradiol (E-2) concentrations (P <= 0.05) and lesser LHCGR, CYP19A1, and HSD3B1 transcript abundances (P <= 0.05). Heifers treated to induce PE had a larger follicle diameter (P = 0.09), greater intra-follicular E-2 concentrations and larger abundances of CYP19A1 mRNA transcript (P <= 0.05) than heifers of the D6 group. Overall, treatment with larger doses of P-4 resulted in lesser abundances of LHCGR, HSD3B1, and CYP19A1 mRNA transcripts; thus, potentially leading to development of smaller dominant follicles and lesser E-2 concentrations.
Descrição
Palavras-chave
LH receptor, Progesterone, Granulosa cell, Nellore heifers
Idioma
Inglês
Como citar
Animal Reproduction Science. Amsterdam: Elsevier, v. 225, 10 p., 2021.
