Efeito isolado ou combinado do Ácido Anacárdico com Paclitaxel na invasividade de células tumorais ovarianas
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2021-07-30
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Universidade Estadual Paulista (Unesp)
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O câncer de ovário é a doença ginecológica que mais mata mulheres no mundo. Este tipo de câncer tende a ser diagnosticado de maneira tardia, devido a sua natureza assintomática, sendo utilizado no tratamento a quimioterápia baseada em taxanos, como o Paclitaxel. Porém, este medicamento vem sofrendo resistência por parte das células neoplásicas ovarianas, comprometendo a recuperação das pacientes. Pesquisas recentes demonstraram que a associação entre quimioterápicos e produtos naturais otimizam a eficácia dos mesmos. Assim, o presente estudo teve como objetivo avaliar o efeito do ácido anacárdico, isolado ou combinado com o agente antineoplásico Paclitaxel, sobre o potencial invasivo de células humanas de câncer de ovário. Para isso, células da linhagem SKOV-3 foram cultivadas e expostas ao Ácido anacárdico e Paclitaxel, de maneira isolada e combinada, analisando alterações morfológicas, a viabilidade celular e migração por inserto transwell. De maneira geral, nosso estudo mostrou que o Ácido anacárdico diminuiu a capacidade migratória das células, assim como afetou a viabilidade celular, tanto isolado quanto em sinergia com o antineoplásico Paclitaxel. Além disso, também identificamos a CXCL6 como um novo potencial alvo terapêutico para o câncer de ovário, através de análises in silico. Dessa forma acreditamos que nossos resultados possam contribuir com pesquisas futuras envolvendo aassociação entre produtos naturais e tratamentos convencionais, particularmente no sucesso da aplicação do Ácido anacárdico no tratamento de câncer de ovário.
Ovarian cancer is the fifth gynecological disease that kills more women in the world. This type of cancer tends to be diagnosed late, due to its asymptomatic nature, being used in the treatment chemotherapy based on taxanes, such as paclitaxel. However, this drug has suffered resistance from ovarian neoplastic cells, compromising the recovery of patients. Recent researches have demonstrated that the association between chemotherapy and natural products optimizes their efficacy. Thus, the present study aimed to evaluate the effect of anacardic acid, alone or combined with the antineoplastic agent paclitaxel, on the invasive potential of human ovarian cancer cells. To this end, cells of the SKOV-3 lineage were cultured and exposed to anacardic acid and paclitaxel, in isolation and synergistically, analyzing morphological changes, cell viability, and migration by Transwell assay. In general, our study showed that anacardic acid decreased the migratory capacity of cells, as well as affected cell viability, both alone and in synergy with the antineoplastic paclitaxel. In addition, we have also identified CXCL6 as a potential new therapeutic target for ovarian cancer through in silico analyses. Thus, we believe that our results can contribute to future research on the association between natural products and conventional treatments, particularly in the successful application of anacardic acid in the treatment of ovarian cancer.
Ovarian cancer is the fifth gynecological disease that kills more women in the world. This type of cancer tends to be diagnosed late, due to its asymptomatic nature, being used in the treatment chemotherapy based on taxanes, such as paclitaxel. However, this drug has suffered resistance from ovarian neoplastic cells, compromising the recovery of patients. Recent researches have demonstrated that the association between chemotherapy and natural products optimizes their efficacy. Thus, the present study aimed to evaluate the effect of anacardic acid, alone or combined with the antineoplastic agent paclitaxel, on the invasive potential of human ovarian cancer cells. To this end, cells of the SKOV-3 lineage were cultured and exposed to anacardic acid and paclitaxel, in isolation and synergistically, analyzing morphological changes, cell viability, and migration by Transwell assay. In general, our study showed that anacardic acid decreased the migratory capacity of cells, as well as affected cell viability, both alone and in synergy with the antineoplastic paclitaxel. In addition, we have also identified CXCL6 as a potential new therapeutic target for ovarian cancer through in silico analyses. Thus, we believe that our results can contribute to future research on the association between natural products and conventional treatments, particularly in the successful application of anacardic acid in the treatment of ovarian cancer.
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Ovários tumores, Paclitaxel, Citocinas, Quimiocinas, Células-tronco neoplásicas, Ácido anacárdico, Chemokine CXCL6