Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse
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Data
2021-08-01
Autores
Kuniyoshi, Tais Mayumi
Nobrega Mendonca, Carlos Miguel
Vieira, Viviane Borges
Robl, Diogo
Gombossy de Melo Franco, Bernadette Dora
Todorov, Svetoslav Dimitrov
Tome, Elisabetta
O'Connor, Paula Mary
Converti, Attilio
Araujo, Welington Luiz
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Editor
Elsevier B.V.
Resumo
Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1. ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg & sdot;L-1 of pure pediocin (purity > 95%). Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers. Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts. This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production. <comment>Superscript/Subscript Available</comment
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Palavras-chave
Sugarcane bagasse, Bacteriocin, Hemicellulose hydrolysate, < em > Pediococcus pentosaceus <, em >
Como citar
Applied Geochemistry. Oxford: Pergamon-elsevier Science Ltd, v. 131, 12 p., 2021.