In vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy
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2011-03-01
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Venturini, James [UNESP]
Soares, Cléverson Teixeira
Belone, Andréa de Faria Fernandes
Barreto, Jaison Antonio
Ura, Somei
Lauris, José Roberto Pereira
Vilani-Moreno, Fátima Regina
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Objective: We investigated the in vitro and skin lesions production of cytokines in non-treated borderline tuberculoid (BT) and borderline lepromatous (BL) patients. Patients and Methods: Seven untreated, non-reactional BT patients and 12 untreated, non-reactional BL patients were studied. Levels of the cytokines IFN-γ, IL-10, TGF-β1 and TNF-α were measured in supernantant of peripheral blood mononuclear cells (PBMC) cultures, stimulated with specific M. leprae antigen (sonicated and whole). The cytokines iNOS, IL-10 and TGF-β1 were detected by immunohistochemistry in skin biopsies. Results: BT patients produced higher levels of IFN-γ than BL patients; iNOS expression in skin lesions was also higher in BT patients. TGF-β1 was detected in more cells in BL patients; IL-10 expression was similar in both groups. There was a negative correlation between iNOS and TGF-β1 expression in skin biopsies, positive correlation between TGF-b1 in skin lesions and bacillary index, as well as positive correlation between iNOS detected in skin biopsies and PBMC IFN- γ production. Conclusions: The BT patients had a mainly a Th1-profile of cytokines in their skin lesions and BL patients had aTh2 profile. © Lepra.
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Leprosy Review, v. 82, n. 1, p. 25-35, 2011.