Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.
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Data
1994-01-01
Autores
Machado, R. Z. [UNESP]
Valadão, C. A. [UNESP]
Melo, W. R. [UNESP]
Alessi, A. C. [UNESP]
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1.We describe the isolation of viable merozoites from erythrocytes infected with Babesia bovis or Babesia bigemina organisms by ammonium chloride lysis. 2. Parasite morphology was examined by both light and transmission electron microscopy. Erythrocyte-free parasites maintain their viability and infectivity, retain their antigenicity and are suitable for use in the indirect fluorescent antibody assay.
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Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], v. 27, n. 11, p. 2591-2598, 1994.