Publicação: Production of β-galactosidase by Trichoderma reesei FTKO-39 in wheat bran: Partial purification of two isozymes
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Trichoderma reesei FTKO-39 grown at 35°C for 5 d on wheat bran supplemented with MgCl2 and lactose as the carbon source produced two isozymes of β-galactosidase: BGT I and BGT II. These isozymes were partially purified on a DEAE-Trisacryl column. Both BGT I and BGT II fractions exhibited optimum activity at 65°C, but the pH optima were 4.0 and 6.5, respectively. The isozymes also showed similar thermal stability. However, BGT I was more stable than BGT II in a pH range of 3.0-10.0. At least two different β-galactosidases are produced by T. reesei, as revealed by the two bands seen on a 6% polyacrylamide gel stained for activity. Copyright © 2006 by Humana Press Inc. All rights of any nature whatsoever reserved.
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β-Galactosidase, Isozymes, Partial purification, Semisolid fermentation, Trichoderma reesei
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Inglês
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Applied Biochemistry and Biotechnology, v. 133, n. 2, p. 163-170, 2006.
