Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results
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The practice of exercise can change biological markers. Thus, the use of saliva to define possible biomarkers has increased with the advances in “omics” techniques. Therefore, this study analyzed the salivary proteomic profile in young adults before and after interval exercise. Five adults, without comorbidities and with normal salivary flow, aged between 20 and 35 years (3 men and 2 women) were selected. The collection of stimulated (SS) and non-stimulated (NSS) saliva was done before (BE) and after (AE) exercise, which consisted of interval exercise with 5 min walking on a treadmill and 5 min at rest, during a total of 40 min. The proteins were extracted individually and processed by label-free proteomics. There was no significant difference between BE and AE salivary flows. For SS, 69 and 60 proteins were found BE (12 exclusives) and AE (3 exclusives), respectively. Ten subunits of immunoglobulins (8 more than twofold), as well as Lysozyme C and Cystatin-S, were increased AE compared to BE. Six hemoglobin’s isoforms were decreased AE compared to BE. For NSS, 76 and 65 proteins were found BE (18 exclusives) and AE (7 exclusives), respectively. Albumin was increased AE, while Basic salivary proline-rich protein 1 and 2 decreased. Apolipoprotein A-I was identified exclusively AE compared to BE. The salivary proteome after interval exercise suggests increase of immunity.