Autocoleta e teste de DNA HPV como método de rastreamento em Mulheres Privadas de Liberdade no Amazonas
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Data
2021-12-15
Autores
Pereira, Hilka Flavia Barra do Espírito Santo Alves
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
As Mulheres Privadas de Liberdade (MPL) apresentam múltiplos fatores de risco para a presença de Papilomavírus Humano (HPV) cervical, oral e anal, sendo que a autocoleta pode ser uma alternativa para o rastreio. Nesse sentido, o objetivo deste estudo foi analisar o rastreio de HPV através da pesquisa por testes moleculares na topografia cérvico-vaginal a partir de autocoleta de MPL no estado do Amazonas,e os casos positivos foram coletadas amostras de cavidade oral e anal . Trata-se de um estudo observacional, transversal, descritivo e analítico de prevalência do diagnóstico de infecção por HPV, do qual participaram 268 MPL no estado do Amazonas, sendo estas submetidas à autocoleta por meio da utilização do dispositivo Coari® e detecção pelo Teste Cobas® 4800 HPV CTNG (Roche®). As participantes com HPV detectável foram submetidas à colpocitologia de meio líquido, imunocitoquímica através da superexpressão da proteína p16INK4a, colposcopia e biópsia, quando indicado com análise histopatológica. Além disso os casos positivos cérvico-vaginal foram submetidas à autocoleta para as cavidades oral e anal, com análise pelo teste HPV 40™. O HPV foi detectado em 66 (24,6%) das mulheres, sendo encontrado o HPV 16 em 12 (4,5%) delas, HPV 18 em três (1,1%) e outros tipos de HPV de alto risco em 51 (19%); resultados inconclusivos totalizaram 21 (7,8%) e negativos em 181 (67,5%) das pacientes.Das 50 mulheres pesquisadas com teste positivo cérvico-vaginal a presença do HPV na amostra oral se deu em 24% das pacientes e anal em 62%. A aceitabilidade e desempenho da autocoleta, como método diagnóstico, pode ser uma alternativa para o rastreio em vários sítios.
Women Deprived of Liberty (MPL) have multiple risk factors for the presence of cervical, oral and anal Human Papillomavirus (HPV), and self-collection can be an alternative for screening. In this sense, the aim of this study was to analyze the HPV screening through research by molecular tests in the cervicovaginal topography from MPL autocollection in the state of Amazonas, and the positive cases were collected samples from the oral and anal cavities. This is an observational, cross-sectional, descriptive and analytical study of the prevalence of the diagnosis of HPV infection, in which 268 MPL participated in the state of Amazonas, which were submitted to self collection through the use of the Coari® device and detection by the Cobas Test ® 4800 HPV CTNG (Roche®). Participants with detectable HPV underwent colpocytology of liquid medium, immunocytochemistry through P16INK4a protein overexpression, colposcopy and biopsy, when indicated with histopathological analysis. In addition, the vaginal cervix positive cases were submitted to self-collection for the oral and anal cavities, with analysis using the HPV 40™ test. HPV was detected in 66 (24.6%) of the women, with HPV 16 being found in 12 (4.5%) of them, HPV 18 in three (1.1%) and other types of high risk of HPV in 51 (19%); inconclusive results totaled 21 (7.8%) and negative in 181 (67.5%) of the patients. The presence of HPV in the oral sample occurred in 24 % of the vaginal cervix patients and in 62% of the anal samples. The acceptability and performance of self-collection, as a diagnostic method, can be an alternative for screening at multiple sites.
Women Deprived of Liberty (MPL) have multiple risk factors for the presence of cervical, oral and anal Human Papillomavirus (HPV), and self-collection can be an alternative for screening. In this sense, the aim of this study was to analyze the HPV screening through research by molecular tests in the cervicovaginal topography from MPL autocollection in the state of Amazonas, and the positive cases were collected samples from the oral and anal cavities. This is an observational, cross-sectional, descriptive and analytical study of the prevalence of the diagnosis of HPV infection, in which 268 MPL participated in the state of Amazonas, which were submitted to self collection through the use of the Coari® device and detection by the Cobas Test ® 4800 HPV CTNG (Roche®). Participants with detectable HPV underwent colpocytology of liquid medium, immunocytochemistry through P16INK4a protein overexpression, colposcopy and biopsy, when indicated with histopathological analysis. In addition, the vaginal cervix positive cases were submitted to self-collection for the oral and anal cavities, with analysis using the HPV 40™ test. HPV was detected in 66 (24.6%) of the women, with HPV 16 being found in 12 (4.5%) of them, HPV 18 in three (1.1%) and other types of high risk of HPV in 51 (19%); inconclusive results totaled 21 (7.8%) and negative in 181 (67.5%) of the patients. The presence of HPV in the oral sample occurred in 24 % of the vaginal cervix patients and in 62% of the anal samples. The acceptability and performance of self-collection, as a diagnostic method, can be an alternative for screening at multiple sites.
Descrição
Palavras-chave
Autocoleta, hpv, Rastreio, Sistema carcerário