Características estruturais e ultraestruturais da língua de camundongos mdx
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Data
2022-05-26
Autores
Tomiate, André Neri [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A distrofia muscular de Duchenne (DMD) consiste em uma patologia ligada ao cromossomo X que acomete humanos e caracteriza-se por ciclo de degeneração e regeneração das fibras musculares, ocasionando na deposição de tecido conjuntivo e adiposo na musculatura. Nos estágios iniciais, o comprometimento motor é característico, entretanto outras regiões também apresentam alterações, como as musculaturas da cavidade oral. Associadas na deglutição alimentar, os diferentes músculos mastigatórios apresentam alterações que causam dificuldades na deglutição. Para o estudo da DMD, o modelo animal mais utilizado é o camundongo mdx, apesar de existir a caracterização da musculatura da língua, as repercussões na mucosa lingual ainda não foram evidenciadas. O objetivo do presente estudo consistiu em descrever as alterações morfológicas estruturais e ultraestruturais do epitélio, lamina própria e musculatura da língua de camundongos mdx. Foram utilizados 40 camundongos com 6 meses de idade organizados em dois grupos: Grupo Controle: n = 20 (C57BL/10) e mdx: n = 20 (C57BL/10mdx). As amostras foram processadas para análises estruturais, tridimensionais e ultraestruturais sob técnicas de microscopia de luz, microscopia eletrônica de varredura e microscopia eletrônica de transmissão. Estavam presentes papilas filiformes cônicas e triangulares, fungiformes, folhadas e valadas, e seus respectivos tecidos conjuntivos associados. As papilas triangulares filiformes apresentaram múltiplas pontas devido a maior desgaste. O epitélio foi caracterizado estratificado em camadas: córnea, granulosa, espinhosa e basal, o grupo mdx apresentou maior espessura nas diferentes regiões do corpo da língua. A lâmina própria possui composição de fibras de colágeno de coloração avermelhada e esverdeada, no grupo mdx, houve maior densidade de fibras colágenas. A região da submucosa apresentou glândulas mucosas e serosas com a presença de colágeno adjacente, aumento da área das glândulas mucosas e diminuição das serosas no grupo mdx. A musculatura dos camundongos mdx foi caracterizada pela grande deposição de colágeno entre as fibras, miocitólise e foco inflamatório. Em conclusão, a língua de camundongos mdx com 6 meses de idade teve os aspectos morfológicos, estruturais e ultraestruturais evidenciados, a distrofia proporcionou alterações indiretas na morfologia na espessura epitelial e desgaste das papilas, e direta na composição da lâmina própria, submucosa e musculatura dos camundongos mdx.
Duchenne muscular dystrophy (DMD) consists of a pathology related to the X chromosome that affects humans and characterized by a cycle of degeneration and regeneration of muscular fiber, causing deposition of connective and adipose tissue on the musculature. In the early stages, the motor commitment is very characteristic; however in other regions also have alterations, as the oral cavity musculature. Associated with food swallowing, the different masticatory muscles present changes that provide difficulties during feeding. For the study of DMD, animal models are used and the mdx mouse is the more common, although there is a characterization of the tongue musculature, aspects of the lingual mucosa have not yet been evidenced. The aim of the study was describe changes in structural and ultrastructural morphological characteristics of the epithelium, lamina propria and tongue musculature of mdx mice. Forty mice with 6 months-old were used, organized into two groups: Control Group: n = 20 (C57bl/10) and mdx: n = 20 (C57bl/10mdx). Samples were processed for structural, tridimensional and ultrastructural analysis under light microscopy, scanning electron microscopy and transmission electron microscopy. Our results showed conical and triangular filiform, fungiform, foliate, and vallate papillae, and its connective tissue cores. The filiform triangular papillae showed multiple tips due to increased wear. The epithelium was characterized stratified into layers: keratinized, granular, spinous and basal, and different regions of the tongue’s body had thicker epithelium in the mdx group. The lamina propria has a composition of reddish and greenish collagen fibers, in the mdx there was a higher density of collagen fibers. The region of submucosa had mucous and serous glands with the presence of adjacent collagen, higher area of mucous glands and lower area of serous glands in mdx group. The musculature of mdx mice was characterized by large collagen deposition between fibers, myocytolysis and inflammatory focus. In conclusion, the tongue of mdx mice with 6 months-age had the morphological, structural and ultrastructural aspects evidenced; dystrophy provided indirect changes in the epithelium thickness and wear of papillae and direct changes in the composition of the lamina propria, submucosa, and musculature of the mdx mice.
Duchenne muscular dystrophy (DMD) consists of a pathology related to the X chromosome that affects humans and characterized by a cycle of degeneration and regeneration of muscular fiber, causing deposition of connective and adipose tissue on the musculature. In the early stages, the motor commitment is very characteristic; however in other regions also have alterations, as the oral cavity musculature. Associated with food swallowing, the different masticatory muscles present changes that provide difficulties during feeding. For the study of DMD, animal models are used and the mdx mouse is the more common, although there is a characterization of the tongue musculature, aspects of the lingual mucosa have not yet been evidenced. The aim of the study was describe changes in structural and ultrastructural morphological characteristics of the epithelium, lamina propria and tongue musculature of mdx mice. Forty mice with 6 months-old were used, organized into two groups: Control Group: n = 20 (C57bl/10) and mdx: n = 20 (C57bl/10mdx). Samples were processed for structural, tridimensional and ultrastructural analysis under light microscopy, scanning electron microscopy and transmission electron microscopy. Our results showed conical and triangular filiform, fungiform, foliate, and vallate papillae, and its connective tissue cores. The filiform triangular papillae showed multiple tips due to increased wear. The epithelium was characterized stratified into layers: keratinized, granular, spinous and basal, and different regions of the tongue’s body had thicker epithelium in the mdx group. The lamina propria has a composition of reddish and greenish collagen fibers, in the mdx there was a higher density of collagen fibers. The region of submucosa had mucous and serous glands with the presence of adjacent collagen, higher area of mucous glands and lower area of serous glands in mdx group. The musculature of mdx mice was characterized by large collagen deposition between fibers, myocytolysis and inflammatory focus. In conclusion, the tongue of mdx mice with 6 months-age had the morphological, structural and ultrastructural aspects evidenced; dystrophy provided indirect changes in the epithelium thickness and wear of papillae and direct changes in the composition of the lamina propria, submucosa, and musculature of the mdx mice.
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Palavras-chave
Língua, Epitélio, Distrofia muscular de Duchenne, Papilas linguais, Tongue, Epithelium, Lingual papillae, Duchenne muscular dystrophy