Características físico-químicas e biológicas do clinker e dos cimentos MTA e MTA Repair HP Angelus
Carregando...
Data
2022-03-31
Autores
Oliveira, Bruna Vasconcellos de
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
Novas formulações de cimentos à base de silicato de cálcio são desenvolvidas. Atualmente, MTA Angelus (MTA) e MTA Repair HP (MTA HP) são compostos a partir do clinker Angelus acrescido de tungstato de cálcio como radiopacificador, sendo manipulados com água destilada (AG) ou água com aditivos (AA). Esta pesquisa experimental avaliou o efeito do radiopacificador e do líquido nas propriedades do clinker (CL), em comparação ao MTA e MTA HP. Tempo de presa, radiopacidade, solubilidade e pH foram avaliados com base na norma ISO 6876/2012. Viabilidade e bioatividade celular também foram avaliadas. Os testes MTT e vermelho neutro foram realizados para avaliação da viabilidade celular e fosfatase alcalina para avaliação da bioatividade após contato com os materiais por 1, 3 e 7 dias. Os dados obtidos foram submetidos aos testes estatísticos ANOVA/Tukey e Bonferroni com nível de significância de 5%. Não houve diferença entre os materiais quanto ao tempo de presa (p>0.05). A radiopacidade de CL+AG e CL+AA foi similar (p>0.05). Não houve diferença entre MTA e MTAHP (p>0.05), que apresentaram maior radiopacidade que CL+AG e CL+AA (p<0.05). Todos os materiais avaliados mostraram ganho de massa, além de apresentarem pH alcalino. Os materiais não foram citotóxicos e apresentaram bioatividade ao 3 e 7 dias (p<0.05). Conclui-se que a manipulação do clinker com água destilada ou água com aditivos e a adição do radiopacificador tungstato de cálcio não interferem nas propriedades biológicas de viabilidade e bioatividade celular do clinker. A presença de tungstato de cálcio proporciona radiopacidade, sem alterar tempo de presa, mantendo a estabilidade dimensional e pH alcalino.
New cement formulations based on calcium silicate are developed. Currently, MTA Angelus (MTA) and MTA Repair HP (MTA HP) are composed from the Angelus clinker plus calcium tungstate as a radiopacifier, being manipulated with distilled water (AG) or water with additives (AA). This experimental research evaluated the effect of radiopacifier and liquid on clinker (CL) properties, compared to MTA and MTA HP. Setting time, radiopacity, solubility and pH were evaluated based on the ISO 6876/2012 standard. Viability and cellular bioactivity were also evaluated. MTT and neutral red tests were performed to evaluate cell viability and alkaline phosphatase to evaluate bioactivity after contact with the materials for 1, 3 and 7 days. The data obtained were submitted to ANOVA/Tukey and Bonferroni statistical tests with a significance level of 5%. There was no difference between the materials in terms of setting time (p>0.05). The radiopacity of CL+AG and CL+AA was similar (p>0.05). There was no difference between MTA and MTAHP (p>0.05), which showed greater radiopacity than CL+AG and CL+AA (p<0.05). All materials evaluated showed mass gain, in addition to presenting alkaline pH. The materials were not cytotoxic and showed bioactivity at 3 and 7 days (p<0.05). It is concluded that the manipulation of the clinker with distilled water or water with additives and the addition of the radiopacifier calcium tungstate do not interfere in the biological properties of viability and cellular bioactivity of the clinker. The presence of calcium tungstate provides radiopacity, without changing the setting time, maintaining dimensional stability and alkaline pH.
New cement formulations based on calcium silicate are developed. Currently, MTA Angelus (MTA) and MTA Repair HP (MTA HP) are composed from the Angelus clinker plus calcium tungstate as a radiopacifier, being manipulated with distilled water (AG) or water with additives (AA). This experimental research evaluated the effect of radiopacifier and liquid on clinker (CL) properties, compared to MTA and MTA HP. Setting time, radiopacity, solubility and pH were evaluated based on the ISO 6876/2012 standard. Viability and cellular bioactivity were also evaluated. MTT and neutral red tests were performed to evaluate cell viability and alkaline phosphatase to evaluate bioactivity after contact with the materials for 1, 3 and 7 days. The data obtained were submitted to ANOVA/Tukey and Bonferroni statistical tests with a significance level of 5%. There was no difference between the materials in terms of setting time (p>0.05). The radiopacity of CL+AG and CL+AA was similar (p>0.05). There was no difference between MTA and MTAHP (p>0.05), which showed greater radiopacity than CL+AG and CL+AA (p<0.05). All materials evaluated showed mass gain, in addition to presenting alkaline pH. The materials were not cytotoxic and showed bioactivity at 3 and 7 days (p<0.05). It is concluded that the manipulation of the clinker with distilled water or water with additives and the addition of the radiopacifier calcium tungstate do not interfere in the biological properties of viability and cellular bioactivity of the clinker. The presence of calcium tungstate provides radiopacity, without changing the setting time, maintaining dimensional stability and alkaline pH.
Descrição
Palavras-chave
Endodontia, Materiais dentários, Fenômenos físicos, Teste de materiais, Endodontics, Dental materials, Physical phenomena, Materials testing