Perfil hematológico e enzimático de frangos de corte suplementados com prebióticos e desafiados com água contaminada com excretas
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Data
2022-07-18
Autores
Silva, Mariana Gonçalves
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Universidade Estadual Paulista (Unesp)
Resumo
O objetivo desta pesquisa foi verificar a influência de diversas formulações prebióticas sobre o perfil hematológico e atividade de enzimas do pâncreas de frangos de corte desafiados com água contaminada com excretas. O experimento foi realizado no galpão experimental para frangos de corte da Faculdade de Ciências Agrarias e Tecnológicas da UNESP - Campus de Dracena, onde foram alojados 900 pintos de corte, machos, com um dia de idade, da linhagem Ross, obtidos em um incubatório comercial. As aves foram distribuídas em um delineamento inteiramente casualizado constituído de seis tratamentos: T1 (Golf®) - ração basal com inclusão de 0,2% de prebiótico misto, composto por FOS (frutooligossacarídeos) e GOS (galactooligossacarídeos); T2 (Golf Premium®) - ração basal com inclusão de 0,15% de prebiótico misto, composto por FOS (frutooligossacarídeos), GOS (galactooligossacarídeos) e betaglucanos; T3 (GlucanMos®) - ração basal com inclusão de 0,2% de prebiótico misto, composto por betaglucanos e MOS (mananoligossacarídeos); T4 (FOS®) - ração basal com inclusão de 0,1% de FOS (frutooligossacarídeos); T5 (GOS®) - ração basal com inclusão de 0,1% de GOS (galactooligossacarídeos); T6 (Avilamicina) - ração basal com inclusão de 0,005% de antibiótico promotor de crescimento. As aves de todos os tratamentos foram desafiadas aos 22 e 35 dias com uma solução confeccionada à base de água e cama aviária na proporção de 4Kg de cama reutilizada para 40L de água. Aos 42 dias de idade, as aves foram amostradas, sacrificadas e realizada a colheita de 3 mL de sangue de cinco aves/tratamento, através de punção da veia braquial. Foram obtidos dados de variáveis hematológicas: hematócrito, hemoglobina e índices de Wintrobe, número de eritrócitos, contagem total e diferencial das células de defesa. Nessa idade, foram também coletados fragmentos do pâncreas de cinco aves/tratamento para determinação da atividade pancreática das enzimas amilase e lipase. Os dados foram submetidos à análise de variância pelo procedimento MIXED e, quando houve efeito significativo, as médias foram comparadas pelo teste de Tukey com 5% de significância. Em ambas analises não houve diferença estatística, porem na analise enzimática pode se observar que os tratamentos utilizando FOS houveram um melhor desempenho.
The objective of this research was to verify the influence of different prebiotic formulations on the hematological profile and activity of enzymes in the pancreas of broilers challenged with water contaminated with excreta. The experiment was carried out in the experimental shed for broilers of the Faculty of Agricultural and Technological Sciences of UNESP - Campus de Dracena, where 900 one-day-old male broiler chicks of the Ross strain were housed in a commercial hatchery. . The birds were distributed in a completely randomized design consisting of six treatments: T1 (Golf®) - basal diet with inclusion of 0.2% of mixed prebiotic, composed of FOS (fructooligosaccharides) and GOS (galactooligosaccharides); T2 (Golf Premium®) - basal diet with 0.15% mixed prebiotic, composed of FOS (fructooligosaccharides), GOS (galactooligosaccharides) and beta-glucans; T3 (GlucanMos®) - basal diet with 0.2% mixed prebiotic, composed of beta-glucans and MOS (mannanoligosaccharides); T4 (FOS®) - basal ration with inclusion of 0.1% of FOS (fructooligosaccharides); T5 (GOS®) - basal diet with inclusion of 0.1% of GOS (galactooligosaccharides); T6 (Avilamycin) - basal diet with 0.005% growth promoting antibiotic. The birds of all treatments were challenged at 22 and 35 days with a solution made from water and poultry litter in the proportion of 4Kg of reused litter to 40L of water. At 42 days of age, the birds were sampled, sacrificed and 3 mL of blood was collected from five birds/treatment, through brachial vein puncture. Data on hematological variables were obtained: hematocrit, hemoglobin and Wintrobe indices, number of erythrocytes, total and differential counts of defense cells. At this age, pancreas fragments were also collected from five birds/treatment to determine the pancreatic activity of amylase and lipase enzymes. Data were submitted to analysis of variance by the MIXED procedure and, when there was a significant effect, the means were compared by Tukey's test with 5% significance. In both analyzes there was no statistical difference, however in the enzymatic analysis it can be observed that the treatments using FOS had a better performance.
The objective of this research was to verify the influence of different prebiotic formulations on the hematological profile and activity of enzymes in the pancreas of broilers challenged with water contaminated with excreta. The experiment was carried out in the experimental shed for broilers of the Faculty of Agricultural and Technological Sciences of UNESP - Campus de Dracena, where 900 one-day-old male broiler chicks of the Ross strain were housed in a commercial hatchery. . The birds were distributed in a completely randomized design consisting of six treatments: T1 (Golf®) - basal diet with inclusion of 0.2% of mixed prebiotic, composed of FOS (fructooligosaccharides) and GOS (galactooligosaccharides); T2 (Golf Premium®) - basal diet with 0.15% mixed prebiotic, composed of FOS (fructooligosaccharides), GOS (galactooligosaccharides) and beta-glucans; T3 (GlucanMos®) - basal diet with 0.2% mixed prebiotic, composed of beta-glucans and MOS (mannanoligosaccharides); T4 (FOS®) - basal ration with inclusion of 0.1% of FOS (fructooligosaccharides); T5 (GOS®) - basal diet with inclusion of 0.1% of GOS (galactooligosaccharides); T6 (Avilamycin) - basal diet with 0.005% growth promoting antibiotic. The birds of all treatments were challenged at 22 and 35 days with a solution made from water and poultry litter in the proportion of 4Kg of reused litter to 40L of water. At 42 days of age, the birds were sampled, sacrificed and 3 mL of blood was collected from five birds/treatment, through brachial vein puncture. Data on hematological variables were obtained: hematocrit, hemoglobin and Wintrobe indices, number of erythrocytes, total and differential counts of defense cells. At this age, pancreas fragments were also collected from five birds/treatment to determine the pancreatic activity of amylase and lipase enzymes. Data were submitted to analysis of variance by the MIXED procedure and, when there was a significant effect, the means were compared by Tukey's test with 5% significance. In both analyzes there was no statistical difference, however in the enzymatic analysis it can be observed that the treatments using FOS had a better performance.
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Palavras-chave
Aves, Frutooligossacarídeos, Galactooligossacarídeos, Imunonutrição, Mananoligossacarídeis, Prebiótico, Poultry, Fructooligosaccharides, Galactooligosaccharides, Immunonutrition, Mannanoligosaccharides, Prebiotic