Atividade inibitória de partículas liberadoras de íons (S-PGR) sobre metaloproteinases ligadas ao colágeno e efeito sobre a degradação da matriz dentinária
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Data
2022-03-29
Autores
Souza, Isabela Guiné de
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Universidade Estadual Paulista (Unesp)
Resumo
Objetivo: Avaliar a atividade inibitória de um eluato produzido a partir de partículas
liberadoras de íons (S-PRG) sobre metaloproteinases de matriz (MMPs) ligadas ao
colágeno e degradação da matriz dentinária. Métodos: Quarenta espécimes de
dentina (0,5 × 2,0 × 5,0 mm) obtidos de molares humanos foram completamente
desmineralizados para produzir espécimes de matriz dentinária (MD). A massa seca
de cada espécime foi mensurada e um ensaio colorimétrico determinou a atividade
inicial total de MMPs, permitindo alocar homogeneamente os espécimes em 4 grupos
de tratamento (n=10/grupo): G1) água ultrapura por 1 min (controle negativo), G2) 2%
digluconato de clorexidina (CHX – controle positivo inibitório) por 1 min, G3) eluato de
partículas S-PRG por 1 min e G4) eluato de partículas S-PRG por 30 min. Após os
tratamentos, a atividade total de MMPs foi reavaliada. Então, os espécimes foram
armazenados em fluido corporal simulado (SBF) a 37 ºC por até 21 dias. A massa
seca foi reavaliada semanalmente. Ao sétimo dia, a degradação da MD foi analisada
pela presença de fragmentos de colágeno (FC) e hidroxiprolina (Hyp) no SBF. As
análises estatísticas foram realizadas com ANOVA/Tukey, ANOVA para medidas
repetidas/Sidak e testes t pareados (α=5%). Resultados: A exposição ao eluato de
S-PRG por 1 min e 30 min reduziu significativamente a atividade de MMPs (p<0,0001).
A exposição ao eluato de partículas S-PRG por 30 min apresentou inibição da
atividade de MMPs semelhante à CHX (p=0,061). Ambos os tratamentos com eluato
de partículas S-PRG e CHX reduziram a liberação de FC (p≤0,007) e Hyp (p<0,046).
Após 21 dias de armazenamento em SBF, espécimes tratados com o eluato de
partículas S-PRG, independentemente do tempo de exposição, apresentaram
redução (p≤0,017) de perda de massa, a qual foi intermediária entre CHX e controle
negativo. Conclusão: O tratamento da dentina desmineralizada com eluato de
partículas S-PRG reduziu a atividade total de MMPs ligadas à matriz e a degradação
da matriz dentinária por até 21 dias.
Objective: To evaluate the inhibitory activity of an eluate produced from ion releasing-filler (S-PRG) on dentin collagen-bound metalloproteinases (MMP) and dentin matrix degradation. Methods: Dentin beams (5 × 2 × 0.5 mm) obtained from human molars, were completely demineralized to produce dentin matrix specimens. The dry mass was measured and a colorimetric assay determined the initial total MMPs activity to establish the allocation of the beams into 4 treatment groups (n=10/group): 1) water for 1 min (negative control), 2) 2% chlorhexidine digluconate (CHX – inhibitor control) for 1 min, 3) S-PRG eluate for 1 min, or 4) 30 min. After treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37ºC for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed by the presence of collagen fragments (CF) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA with Tukey’s post-hoc, paired t-tests, and RM-ANOVA with Sidak’s post-hoc (α=5%). Results: S-PRG eluate exposure for 1 min and 30 min significantly (p<0.0001) reduced MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition similar to CHX (p=0.061). S-PRG and CHX decreased CF (p≤0.007) and Hyp (p<0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p≤0.017) loss of mass, intermediate between CHX and control. Conclusion: Treating demineralized dentin with S-PRG eluate reduced matrix-bound MMP activity and decreased matrix degradation for up to 21 days.
Objective: To evaluate the inhibitory activity of an eluate produced from ion releasing-filler (S-PRG) on dentin collagen-bound metalloproteinases (MMP) and dentin matrix degradation. Methods: Dentin beams (5 × 2 × 0.5 mm) obtained from human molars, were completely demineralized to produce dentin matrix specimens. The dry mass was measured and a colorimetric assay determined the initial total MMPs activity to establish the allocation of the beams into 4 treatment groups (n=10/group): 1) water for 1 min (negative control), 2) 2% chlorhexidine digluconate (CHX – inhibitor control) for 1 min, 3) S-PRG eluate for 1 min, or 4) 30 min. After treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37ºC for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed by the presence of collagen fragments (CF) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA with Tukey’s post-hoc, paired t-tests, and RM-ANOVA with Sidak’s post-hoc (α=5%). Results: S-PRG eluate exposure for 1 min and 30 min significantly (p<0.0001) reduced MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition similar to CHX (p=0.061). S-PRG and CHX decreased CF (p≤0.007) and Hyp (p<0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p≤0.017) loss of mass, intermediate between CHX and control. Conclusion: Treating demineralized dentin with S-PRG eluate reduced matrix-bound MMP activity and decreased matrix degradation for up to 21 days.
Descrição
Palavras-chave
Dentina, Colágeno, Hidroxiprolina, Metaloproteinases da Matriz, Íons, Dentin, Collagen, Hydroxyproline, Matrix metalloproteinases, Ions