Isolamento e genotipagem de bartonella henselae em amostras de sangue de gatos (felis catus) do estado de goiás
Carregando...
Data
2022-07-30
Autores
Dias, Clara Morato [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
Bartonella henselae é um patógeno zoonótico responsável por ocasionar a Doença da Arranhadura do Gato (DAG) e outras manifestações clínicas em humanos. Os gatos domésticos são os principais reservatórios dessa espécie de bactéria e estudos prévios sugeriram que determinados genótipos de B. henselae podem ser mais associados a infecções humanas. O presente estudo objetivou genotipar isolados de B. henselae a partir de amostras sanguíneas de gatos domésticos do estado de Goiás, centro-oeste do Brasil. A associação de PCR em tempo real quantitativa (qPCR) baseada no gene nuoG de Bartonella spp. das amostras sanguíneas, antes e após incubação em meio líquido de pré-enriquecimento (BAPGM) e isolamento em ágar chocolate, mostraram frequência de positividade de 43% (43/100) para Bartonella spp. Doze isolados de B. henselae obtidos em ágar chocolate a partir de amostras sanguíneas de seis gatos (dois isolados de cada animal) foram caracterizados por Multi-locus Sequencing Typing (MLST) e revelaram pertencer às Sequence Types ST1 e ST5. Um dos gatos (1/6) apresentou as duas STs, demonstrando que gatos domésticos podem ser coinfectados com diferentes variantes da bactéria. As STs detectadas neste estudo distribuem-se mundialmente e já foram detectadas em humanos com manifestações clínicas por bartonelose. O presente trabalho traz a primeira detecção das variantes zoonóticas ST1 e ST5 de B. henselae em gatos domésticos no Brasil.
Bartonella henselae is a zoonotic pathogen responsible for causing Cat Scratch Disease (CAD) and other clinical manifestations in humans. Domestic cats are the main reservoir of this species of bacteria and previous studies have suggested that certain genotypes of B. henselae may be more associated with human infections. The present study aimed to genotype B. henselae isolates from blood samples from domestic cats in the state of Goiás, midwestern Brazil. The association of quantitative real-time PCR (qPCR) based on the nuoG gene from Bartonella spp. of blood samples, before and after incubation in pre-enrichment liquid medium (BAPGM) and isolation on chocolate agar, showed a positivity frequency of 43% (43/100) for Bartonella spp. Twelve B. henselae isolates obtained on agar chocolate from blood samples from six cats (two isolates from each animal) were characterized by Multi-locus Sequencing Typing (MLST) and revealed to belong to Sequence Types ST1 and ST5. One of the cats (1/6) presented both STs, demonstrating that domestic cats can be coinfected with different variants of the bacteria. The STs detected in this study are distributed worldwide and have already been detected in humans with clinical manifestations of bartonellosis. This is the first reporto f the zoonotic variants ST1 and ST5 of B. henselae in domestic cats from Brazil.
Bartonella henselae is a zoonotic pathogen responsible for causing Cat Scratch Disease (CAD) and other clinical manifestations in humans. Domestic cats are the main reservoir of this species of bacteria and previous studies have suggested that certain genotypes of B. henselae may be more associated with human infections. The present study aimed to genotype B. henselae isolates from blood samples from domestic cats in the state of Goiás, midwestern Brazil. The association of quantitative real-time PCR (qPCR) based on the nuoG gene from Bartonella spp. of blood samples, before and after incubation in pre-enrichment liquid medium (BAPGM) and isolation on chocolate agar, showed a positivity frequency of 43% (43/100) for Bartonella spp. Twelve B. henselae isolates obtained on agar chocolate from blood samples from six cats (two isolates from each animal) were characterized by Multi-locus Sequencing Typing (MLST) and revealed to belong to Sequence Types ST1 and ST5. One of the cats (1/6) presented both STs, demonstrating that domestic cats can be coinfected with different variants of the bacteria. The STs detected in this study are distributed worldwide and have already been detected in humans with clinical manifestations of bartonellosis. This is the first reporto f the zoonotic variants ST1 and ST5 of B. henselae in domestic cats from Brazil.
Descrição
Palavras-chave
Bartonelose, Doença da Arranhadura do Gato, Relação hospedeiro-parasito, Gatos, Patologia