Potencial de isolados do gênero burkholderia sp. Para o controle da diatraea saccharalis (fabricius, 1794), spodoptera frugiperda (je smith, 1797) e de fungos fitopatogênicos
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Data
2022-10-05
Autores
Feitosa, Aparecida Gomes
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O presente trabalho teve como finalidade a prospecção por outras fontes de controle que possam minimizar o ataque de pragas e fitopatógenos causadores de doenças nas plantas. Sendo assim, obtidos um total de 26 isolados bacterianos a partir de amostras, vegetal, solo de área de rio e solo de área de floresta. Avaliados quanto a sua atividade inseticida frente as lagartas neonatas de Diatraea saccharalis e Spodoptera frugiperda. Os 26 isolados também foram testados quanto a atividade fungicida aos fitopatógenos Didymella sp. Colletotrichum sp. e Fusarium sp. Após os testes foram selecionados dois isolados, sendo o primeiro, “isolado 06”, que após sequenciamento parcial do gene 16S rRNA foi possível identifica-lo como sendo do gênero Burkholderia, se aproximando da espécie Burkholderia thailandensis, este apresentou atividade inseticida, foi notado que as lagartas tratadas com esse isolado apresentaram redução no seu peso e/ou crescimento quando comparadas as do controle, mas esta atividade não foi vista nas lagartas que foram tratadas com sobrenadante aquecido do isolado. O isolado que apresentou atividade aos fungos fitopatogênicos, “isolado 26”, que após sequenciamento parcial do gene 16S rRNA foi possível identifica-lo como sendo também do gênero Burkholderia, apresentou halo de inibição ao crescimento dos fungos Didymella sp. Colletotrichum sp. e Fusarium sp., mas não foi observada esta atividade nas placas que estavam com alíquotas do sobrenadante aquecido do isolado. Portanto, notado que em ambos experimentos, na avaliação térmica, a atividade bioinseticida e biofungicida não se mantiveram o que sugerimos preliminarmente tratar-se de um composto sensível ao calor podendo ser uma proteína produzida pelos isolados.
The purpose of this work was to search for other sources of control that can minimize the attack of pests and phytopathogens that cause diseases in plants. Therefore, Spodoptera frugiperda cat erpillars were used as “baits” in the soil, obtaining a total of 26 bacterial isolates and these were evaluated for their insecticidal activity against the neonatal caterpillars of Diatraea saccharalis and Spodoptera frugiperda. The 26 isolates were also t ested for fungicidal activity against the phytopathogens Didymella sp. Colletotrichum sp. and Fusarium sp. After the tests, two isolates were selected, the first being "isolate 06", which after partial sequencing of the 16S rRNA gene it was possible to ide ntify it as belonging to the genus Burkholderia, approaching the species Burkholderia thailandensis, this showed insecticidal activity, it was noticed that the caterpillars treated with this isolate had a reduction in their weight and/or growth when compar ed to the control, but this activity was not seen in the caterpillars that were treated with the warmed supernatant of the isolate. The isolate that showed activity to the phytopathogenic fungi, “isolate 26”, which after partial sequencing of the 16S rRNA gene it was possible to identify it as being also of the Burkholderia genus, showed a halo of inhibition to the growth of the Didymella sp. Colletotrichum sp. and Fusarium sp., but this activity was not observed in the plates that had aliquots of the warme d supernatant of the isolate. Therefore, in both experiments, it was seen that in the thermal evaluation, the bioinsecticide and biofungicide activity were not maintained, which we suggest preliminarily, that it is a heatsensitive compound, which may be a produced by the isolates. Keywords: protein Bioinsecticide, biofungicide, cellular extracts and active compound
The purpose of this work was to search for other sources of control that can minimize the attack of pests and phytopathogens that cause diseases in plants. Therefore, Spodoptera frugiperda cat erpillars were used as “baits” in the soil, obtaining a total of 26 bacterial isolates and these were evaluated for their insecticidal activity against the neonatal caterpillars of Diatraea saccharalis and Spodoptera frugiperda. The 26 isolates were also t ested for fungicidal activity against the phytopathogens Didymella sp. Colletotrichum sp. and Fusarium sp. After the tests, two isolates were selected, the first being "isolate 06", which after partial sequencing of the 16S rRNA gene it was possible to ide ntify it as belonging to the genus Burkholderia, approaching the species Burkholderia thailandensis, this showed insecticidal activity, it was noticed that the caterpillars treated with this isolate had a reduction in their weight and/or growth when compar ed to the control, but this activity was not seen in the caterpillars that were treated with the warmed supernatant of the isolate. The isolate that showed activity to the phytopathogenic fungi, “isolate 26”, which after partial sequencing of the 16S rRNA gene it was possible to identify it as being also of the Burkholderia genus, showed a halo of inhibition to the growth of the Didymella sp. Colletotrichum sp. and Fusarium sp., but this activity was not observed in the plates that had aliquots of the warme d supernatant of the isolate. Therefore, in both experiments, it was seen that in the thermal evaluation, the bioinsecticide and biofungicide activity were not maintained, which we suggest preliminarily, that it is a heatsensitive compound, which may be a produced by the isolates. Keywords: protein Bioinsecticide, biofungicide, cellular extracts and active compound
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Palavras-chave
Composto ativo, Bioinseticidas, Agricultura doenças e pragas, Fungicidas