Detecção dos Equus caballus papillomavirus em amostras de placa aural equina
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Data
2023-01-27
Autores
Bomberger, Cristiana Raach
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A placa aural é uma doença cosmopolita, caracterizada por lesões nodulares na pele que raramente regridem espontaneamente. Foram descritos nove Equus caballus papillomavirus (EcPVs), desses, apenas os EcPVs 1, 3, 4, 5 e 6 foram associados à placa aural no Brasil, ocorrendo de forma isolada ou em coinfecção. Os EcPVs 8 e 9 foram os últimos papilomavírus equinos identificados, por isso há poucas descrições sobre suas características de manifestação clínica e sua associação à placa aural não foi avaliada. O objetivo desse estudo foi verificar a presença do DNA dos EcPV 8 e 9, por PCR, em amostras de placa aural de equinos de diferentes regiões do Brasil. No total, 137 amostras foram utilizadas, das quais 108 foram previamente avaliadas quanto a presença do DNA dos EcPVs 1 – 7 (Mira et al., 2018). As demais amostras, foram coletadas para este estudo (n = 29), e avaliadas quanto a presença dos EcPVs 1 - 9. Foram utilizados primers previamente descritos para as PCRs dos EcPVs 1 – 8, enquanto para o EcPV 9, foram sintetizados primers específicos. Para os EcPVs não detectados em nossas amostras, foram sintetizados mini-genes e utilizados como controle positivo nas reações de PCR. À histopatologia, hiperqueratose, hipomelanose e hiperplasia epidémica foram os principais achados. O DNA dos EcPVs 2, 7, 8 e 9 não foi detectado nas amostras avaliadas. Das amostras testadas para os EcPVs 1 – 9, foram identificados os EcPVs 1, 3, 4, 5 e 6, presentes de forma isolada ou em coinfecção, o EcPV 6 foi o tipo viral mais prevalente nas amostras. Não foi observada relação entre o tipo viral detectado e as características clínicas das lesões. Concluímos que os EcPVs 2, 7, 8 e 9 não tiveram associação com lesões de placa aural analisadas e, podem não estar relacionados à etiologia desta enfermidade no Brasil. A utilização de mini-genes foi fundamental para validar as técnicas de PCR e a sensibilidade dos primers utilizados.
Aural plaque is a cosmopolitan disease, characterized by nodular lesions on the skin that rarely regress spontaneously. Nine Equus Caballus papillomavirus (ECPVs), have been described, of these, only the EcPVs 1, 3, 4, 5 and 6 were associated with aural plaques in Brazil, occurring in isolation or in coinfection. The EcPVs 8 and 9 were the last papilomavirus identified, so there are few descriptions about clinical manifestation features and association with aural plaques were not evaluated. The aim of this study was to verify the DNA presence of EcPVs 8 and 9 by PCR in aural plaques samples of horses from different regions of Brazil. A total of 137 samples were used, of these samples, 108 were previously evaluated for the presence of EcPVs 1 – 7 (Mira et al., 2018). The other samples collected for this study (n = 29), and evaluated for the presence of EcPVs 1 – 9. Primers previously described were used for PCR of EcPVs 1 – 8, while for the EcPV 9, specific primers were synthesized. For the EcPVs not detected in our samples, mini-genes were used as a positive control in the PCR reactions. hyperkeratosis, hypomelanosis and epidemic hyperplasia were the main histopathological findings. The DNA of the EcPVs 2, 7, 8 e 9 was not identified in the analyzed samples. Of the samples tested for the EcPVs 1 – 9, the EcPVs 1, 3, 4, 5 e 6 were identified, in isolated form or in coinfection, the EcPV 6 was the most prevalent viral type in the samples. No relationship was observed between the viral type detected and the clinical lesions features. In conclusion, the EcPVs 2, 7, 8 e 9 was no association with the aural plaque samples analyzed and, therefore, are not associated with the etiology of this disease in Brazil. The use of mini-genes was essential to validate the PCR reactions and the primers sensitivity used in this work.
Aural plaque is a cosmopolitan disease, characterized by nodular lesions on the skin that rarely regress spontaneously. Nine Equus Caballus papillomavirus (ECPVs), have been described, of these, only the EcPVs 1, 3, 4, 5 and 6 were associated with aural plaques in Brazil, occurring in isolation or in coinfection. The EcPVs 8 and 9 were the last papilomavirus identified, so there are few descriptions about clinical manifestation features and association with aural plaques were not evaluated. The aim of this study was to verify the DNA presence of EcPVs 8 and 9 by PCR in aural plaques samples of horses from different regions of Brazil. A total of 137 samples were used, of these samples, 108 were previously evaluated for the presence of EcPVs 1 – 7 (Mira et al., 2018). The other samples collected for this study (n = 29), and evaluated for the presence of EcPVs 1 – 9. Primers previously described were used for PCR of EcPVs 1 – 8, while for the EcPV 9, specific primers were synthesized. For the EcPVs not detected in our samples, mini-genes were used as a positive control in the PCR reactions. hyperkeratosis, hypomelanosis and epidemic hyperplasia were the main histopathological findings. The DNA of the EcPVs 2, 7, 8 e 9 was not identified in the analyzed samples. Of the samples tested for the EcPVs 1 – 9, the EcPVs 1, 3, 4, 5 e 6 were identified, in isolated form or in coinfection, the EcPV 6 was the most prevalent viral type in the samples. No relationship was observed between the viral type detected and the clinical lesions features. In conclusion, the EcPVs 2, 7, 8 e 9 was no association with the aural plaque samples analyzed and, therefore, are not associated with the etiology of this disease in Brazil. The use of mini-genes was essential to validate the PCR reactions and the primers sensitivity used in this work.
Descrição
Palavras-chave
Cavalo, EcPV, Papilomatose, PCR, Doença por papilomavírus, Reação em cadeia da polimerase, Papiloma