Esterilização videoassistida pela técnica de oclusão vascular ovariana com excisão parcial dos ovários e folículos com análise de tomografia computadorizada e ressonância magnética em Trachemys scripta elegans
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Data
2022-12-02
Autores
Baselly-Cueva, Luis Orlando
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Universidade Estadual Paulista (Unesp)
Resumo
A Trachemys scripta elegans é considerada uma espécies exóticas invasoras devido à sua eficiência reprodutiva e adaptação ao ambiente visando evitar a eutanásia ou soltura em habitats indesejados, afetando negativamente as populações nativas, o objetivo deste estudo foi realizar a esterilização pela técnica de oclusão com excisão parcial bilateral dos ovários e folículos, utilizando o nó de Miller´s modificado como ligadura transfixante extracorpórea por meio de vídeo- cirurgia assistida como método de controle reprodutivo. Vinte e duas fêmeas, da espécie Trachemys scripta elegans, divididas em dois grupos (n = 11). No grupo 1 foi realizado exame laboratorial bioquímico e posterior exame de tomografia computadorizada com contraste (TCC); para o grupo 2, foram realizados hemograma e exames bioquímicos, seguidos de ressonância magnética (RM). As imagens de TCC e RM foram usadas para planejamento pré e pós operatório. Os 22 animais foram anestesiados com anestesia balanceada multimodal reversível. O tempo da aplicação da técnica cirúrgica foi medida e dividida em dos grupos segundo o grau de complexidade das afecções celomáticas internas. Após 90, 120 e 150 dias do pós-operatório, uma nova cirurgia foi realizada aleatoriamente, para extração dos ovários remanescentes que foram deixados anteriormente para análise histológica. A histologia mostrou necrose, folículos atrésicos e células inflamatórias aos 90 dias; aos 120 dias houve perda de estrutura tecidual, inflamação e remodelação com presença de tecido conjuntivo; e aos 150 dias algumas estruturas foliculares mineralizadas e tecido cicatricial estavam presentes, comprovando assim que a técnica de esterilização por oclusão videoassistida foi eficiente nesta espécie.
Trachemys scripta elegans is considered an invasive exotic species due to its reproductive efficiency and adaptation to the environment in order to avoid euthanasia or release in unwanted habitats negatively affecting native populations the aim of this study was perform sterilization using the occlusion technique with bilateral partial excision of the ovaries and follicles using a modified Miller´s knot as an extracorporeal transfixing ligature by means of video-assisted surgery as a method of reproductive control. Twenty-two females of the species Trachemys scripta elegans, divided into two groups (n = 11). In group 1 it was biochemical laboratory examination was performed and subsequent computed tomography examination with contrast (CECT); for group 2, blood count and biochemical tests were performed, followed by magnetic resonance imaging (MRI). CECT and MRI images were used to pre and postoperative planning. The 22 animals were anesthetized with anesthesia balanced multimodal reversible. The time of application of the surgical technique was measured and divided into two groups according to the degree of complexity of the internal coelomic affections. After 90, 120 and 150 days after surgery a new surgery was randomly performed, for extracting the remaining ovaries that were previously left for analysis histological. Histology showed necrosis atretic follicles and inflammatory cells at 90 days; at 120 days there was loss of tissue structure inflammation and remodeling with the presence of connective tissue; and at 150 days some mineralized follicular structures and tissue scarring were present, thus proving that the sterilization technique by occlusion video-assisted was efficient in this species.
Trachemys scripta elegans is considered an invasive exotic species due to its reproductive efficiency and adaptation to the environment in order to avoid euthanasia or release in unwanted habitats negatively affecting native populations the aim of this study was perform sterilization using the occlusion technique with bilateral partial excision of the ovaries and follicles using a modified Miller´s knot as an extracorporeal transfixing ligature by means of video-assisted surgery as a method of reproductive control. Twenty-two females of the species Trachemys scripta elegans, divided into two groups (n = 11). In group 1 it was biochemical laboratory examination was performed and subsequent computed tomography examination with contrast (CECT); for group 2, blood count and biochemical tests were performed, followed by magnetic resonance imaging (MRI). CECT and MRI images were used to pre and postoperative planning. The 22 animals were anesthetized with anesthesia balanced multimodal reversible. The time of application of the surgical technique was measured and divided into two groups according to the degree of complexity of the internal coelomic affections. After 90, 120 and 150 days after surgery a new surgery was randomly performed, for extracting the remaining ovaries that were previously left for analysis histological. Histology showed necrosis atretic follicles and inflammatory cells at 90 days; at 120 days there was loss of tissue structure inflammation and remodeling with the presence of connective tissue; and at 150 days some mineralized follicular structures and tissue scarring were present, thus proving that the sterilization technique by occlusion video-assisted was efficient in this species.
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Anestesia, cágado, cirurgia minimamente invasiva, complexo arteriovenoso ovariano, Histologia, imagens, oclusão, ovários, videocirurgia assistida, Anesthesia, Tartarugas, Cirurgia laparoscópica, Cirurgia vídeoassistida, Tomografia, Ressonância magnética