Expressão protéica diferencial entre plântulas apomíticas e zigóticas de citros
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Data
2004-04-01
Autores
Silva, Cristina Lacerda Soares Petrarolha [UNESP]
Machado, Marcos Antônio
Lemos, Eliana Gertrudes Macedo [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Fruticultura
Resumo
Existem evidências do envolvimento de um ou poucos genes controlando o processo apomítico em citros; entretanto, a identidade destes genes, seus produtos e suas formas de atuação ainda não foram elucidados. Sendo as proteínas o produto final da expressão gênica, o processo apomítico pode ser investigado através do estudo da expressão protéica. A técnica da eletroforese bidimensional de proteínas foi empregada nesta pesquisa, objetivando a comparação de perfis protéicos de plântulas apomíticas e zigóticas de citros obtidas a partir de cruzamento entre Citrus sinensis L. Osb. cv. Pêra e Poncirus trifoliata cv. Rubidoux. Observou-se, nestes perfis bidimensionais, a ocorrência de três classes de proteínas: a primeira presente em ambos os perfis, a segunda exclusiva do perfil apomítico e a terceira exclusiva do perfil zigótico. Dentro da classe de proteínas compartilhada por ambos os perfis, observaram-se diferenças no nível de expressão entre zigóticos e apomíticos. A classe protéica exclusiva do perfil zigótico, provavelmente, está relacionada à expressão de genes herdados do parental masculino. Já dentre as proteínas exclusivas do perfil apomítico, podem estar proteínas relacionadas ao processo apomítico. O peso molecular e o ponto isoelétrico destas proteínas de expressão diferencial foram estimados e constituem, então, um banco de dados para futuros trabalhos de purificação e seqüenciamento das mesmas.
There are evidences that one or a few genes only regulate the apomictic process in citrus. However, the genes identities, their products and mode of action were not elucidated yet. As proteins are the final products of gene expression, the investigation of protein expression can lead to a better understanding of the apomictic process. In this research, the bidimensional protein electrophoresis technique was used to compare protein profiles from apomictic and zygotic seedlings of citrus. Three classes of proteins were observed based on bidimensional profiles: a) those observed in either one of the profiles, b) those exclusive to the apomictic profile, and c) those exclusive to the zygotic profile within that class of proteins shared by both profiles. Differences in the expression level were observed for apomitic or zygotic seedlings. The class of protein exclusive to the zygotic profile is probably related to the expression of genes inherited from the male parental. The proteins detected in the apomictic profile could be related to the apomictic process itself. The molecular weight and the isoelectric point of these differentially expressed proteins were also estimated and probably constitute an important data base for future work on purification and sequencing of these proteins.
There are evidences that one or a few genes only regulate the apomictic process in citrus. However, the genes identities, their products and mode of action were not elucidated yet. As proteins are the final products of gene expression, the investigation of protein expression can lead to a better understanding of the apomictic process. In this research, the bidimensional protein electrophoresis technique was used to compare protein profiles from apomictic and zygotic seedlings of citrus. Three classes of proteins were observed based on bidimensional profiles: a) those observed in either one of the profiles, b) those exclusive to the apomictic profile, and c) those exclusive to the zygotic profile within that class of proteins shared by both profiles. Differences in the expression level were observed for apomitic or zygotic seedlings. The class of protein exclusive to the zygotic profile is probably related to the expression of genes inherited from the male parental. The proteins detected in the apomictic profile could be related to the apomictic process itself. The molecular weight and the isoelectric point of these differentially expressed proteins were also estimated and probably constitute an important data base for future work on purification and sequencing of these proteins.
Descrição
Palavras-chave
apomixia, citros, proteína, eletroforese bidimensional, RAPD, apomixis, citrus, protein, bidimensional electrophoresis, RAPD
Como citar
Revista Brasileira de Fruticultura. Sociedade Brasileira de Fruticultura, v. 26, n. 1, p. 1-4, 2004.