Isoenzymatic polymorphism in Citrus spp. and Poncirus trifoliata (L.) Raf. (Rutaceae)
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Data
2000-03-01
Autores
Novelli, Valdenice Moreira [UNESP]
Machado, Marcos Antonio
Lopes, Catalina Romero [UNESP]
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Sociedade Brasileira de Genética
Resumo
A análise do polimorfismo isoenzimático foi usada para determinar a variabilidade genética entre espécies e híbridos de Citrus spp. e um acesso da espécie Poncirus trifoliata (L.) Raf. Dez diferentes sistemas enzimáticos foram analisados, incluindo aspartato aminotransferase (AAT), fosfatase ácida (ACP), leucina aminopeptidase (LAP), 6-fosfogluconato desidrogenase (6-PGD), isocitrato desidrogenase (IDH), fosfoglucose isomerase (PGI), fosfoglucomutase (PGM), diaforase (DIA), shiquimato desidrogenase (SKD) e peroxidase (PRX). Um total de 20 locos e 48 alelos foram identificados. Os cultivares de laranja doce (C. sinensis (L.) Osbeck) apresentaram um grande número de locos heterozigotos, mas similares entre eles, com exceção dos cultivares Westin e Lima graúda. Os cultivares de mandarim (C. reticulata Blanco) apresentaram diferentes padrões entre eles, enquanto que Poncirus trifoliata (L.) Raf. apresentou elevada diferenciação em relação a todas as espécies de Citrus e híbridos. Fenótipos exclusivos foram observados em alguns sistemas enzimáticos, sendo encontrados padrões similares entre os híbridos interespecíficos e seus possíveis parentais.
Isoenzymatic polymorphism analysis was used to determine genetic variability among species and hybrids of Citrus spp. and one accession of Poncirus trifoliata (L.) Raf. Ten enzymatic systems aspartate aminotransferase (AAT), acid phosphatase (ACP), leucine aminopeptidase (LAP), 6-phosphogluconate dehydrogenase (6-PGD), isocitrate dehydrogenase (IDH), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), diaphorase (DIA), shikimate dehydrogenase (SKD) and peroxidase (PRX) were analyzed. Twenty loci and 48 alleles were identified. Sweet orange cultivars (C. sinensis (L). Osbeck) showed the highest polymorphism with the largest number of heterozygous loci, although the alleles of those loci were the same in all cultivars, with the exception of Westin and Lima graúda. Mandarins (C. reticulata Blanco) exhibited diverse patterns, whereas Poncirus trifoliata (L.) Raf. showed high variability with all Citrus species and hybrids. Exclusive phenotypes were observed in some enzymatic systems, and similar patterns were found among interspecific hybrids and their putative parents.
Isoenzymatic polymorphism analysis was used to determine genetic variability among species and hybrids of Citrus spp. and one accession of Poncirus trifoliata (L.) Raf. Ten enzymatic systems aspartate aminotransferase (AAT), acid phosphatase (ACP), leucine aminopeptidase (LAP), 6-phosphogluconate dehydrogenase (6-PGD), isocitrate dehydrogenase (IDH), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), diaphorase (DIA), shikimate dehydrogenase (SKD) and peroxidase (PRX) were analyzed. Twenty loci and 48 alleles were identified. Sweet orange cultivars (C. sinensis (L). Osbeck) showed the highest polymorphism with the largest number of heterozygous loci, although the alleles of those loci were the same in all cultivars, with the exception of Westin and Lima graúda. Mandarins (C. reticulata Blanco) exhibited diverse patterns, whereas Poncirus trifoliata (L.) Raf. showed high variability with all Citrus species and hybrids. Exclusive phenotypes were observed in some enzymatic systems, and similar patterns were found among interspecific hybrids and their putative parents.
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Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 23, n. 1, p. 163-168, 2000.