Agarose gel electrophoresis of cerebrospinal fluid proteins of dogs after sample concentration using a membrane microconcentrator technique
Nenhuma Miniatura disponível
Data
2007-03-01
Autores
Velasque Gama, Fernanda Gomes
Santana, Aureo Evangelista
de Campos Filho, Eugenio
da Silva Nogueira, Claudia Aparecida
Título da Revista
ISSN da Revista
Título de Volume
Editor
Amer Soc Veterinary Clinical Pathology
Resumo
Background: Cerebrospinal fluid (CSF) is produced in the cerebral ventricles through ultrafiltration of plasma and active transport mechanisms. Evaluation of proteins in CSF may provide important information about the production of immunoglobulins within the central nervous system as well as possible disturbances in the blood-brain barrier. Objective: the objective of this study was to measure the concentration and fractions of protein in CSF samples using a membrane microconcentrator technique followed by electrophoresis, and to compare the protein fractions obtained with those in serum. Methods: CSF samples from 3 healthy dogs and 3 dogs with canine distemper virus infection were concentrated using a membrane microconcentrator having a 0.5 to 30,000 d nominal molecular weight limit (Ultrafree, Millipore, Billerica, MA, USA). Protein concentration was determined before and after concentration. Agarose gel electrophoresis was done on concentrated CSF samples, serum, and serial dilutions of one of the CSF samples. Results: Electrophoretic bands were clearly identified in densitometer tracings in CSF samples with protein concentrations as low as 1.3 g/dL. The higher CSF protein concentration in dogs with distemper was mainly the result of increased albumin concentration. Conclusion: the microconcentrating method used in this study enables characterization of the main protein fractions in CSF by routine electrophoresis and may be useful for interpreting the underlying cause of changes in CSF protein concentrations
Descrição
Palavras-chave
agarose gel, cerebrospinal fluid, dog, electrophoresis, globulins, protein
Como citar
Veterinary Clinical Pathology. Baton Rouge: Amer Soc Veterinary Clinical Pathology, v. 36, n. 1, p. 85-88, 2007.