Screening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L)
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Data
2004-03-01
Autores
de Assis, S. A.
Fernandes, P.
Ferreira, B. S.
Cabral, JMS
Oliveira, OMMF
Título da Revista
ISSN da Revista
Título de Volume
Editor
Wiley-Blackwell
Resumo
A partially purified extract of pectinmethylesterase (PME) from acerola fruit was immobilized on various supports: glass, celite, chrysotile, agarose, concanavalin A Sepharose 4B, egg shell, polyacrylamide and gelatin. In addition, reticulation with glutaraldehyde was assessed, as well as the use of gelatin in the presence of celite, glass and silica. The highest immobilization yields were obtained when the pectinmethylesterase was immobilized in concanavalin A Sepharose 4B (81.7%) and in gelatin-water (78.0%). (C) 2004 Society of Chemical Industry.
Descrição
Palavras-chave
acerola, pectinmethylesterase, immobilization, kinetics characterization
Como citar
Journal of Chemical Technology and Biotechnology. Chichester: John Wiley & Sons Ltd, v. 79, n. 3, p. 277-280, 2004.