Analysis of the serological variability of Lettuce mosaic virus using monoclonal antibodies and surface plasmon resonance technology

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Data

2007-09-01

Autores

Candresse, Thierry
Lot, Herve
German-Retana, Sylvie
Krause-Sakate, Renate
Thomas, John
Souche, Sylvie
Delaunay, Thierry
Lanneau, Maryvonne
Le Gall, Olivier

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Soc General Microbiology

Resumo

A panel of 19 monoclonal antibodies (mAbs) was used to study the immunological variability of Lettuce mosaic virus (LMV), a member of the genus Potyvirus, and to perform a first epitope characterization of this virus. Based on their specificity of recognition against a panel of 15 LMV isolates, the mAbs could be clustered in seven reactivity groups. Surface plasmon resonance analysis indicated the presence, on the LMV particles, of at least five independent recognition/ binding regions, correlating with the seven mAbs reactivity groups. The results demonstrate that LMV shows significant serological variability and shed light on the LMV epitope structure. The various mAbs should prove a new and efficient tool for LIVIV diagnostic and field epidemiology studies.

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Journal of General Virology. Reading: Soc General Microbiology, v. 88, p. 2605-2610, 2007.