Indirect fluorescent test for detection of anti-Paracoccidioides brasiliensis antibodies using coated bentonite particles as antigen
MetadataShow full item record
An indirect fluorescent test was developed for detecting antibodies to Paracoccidioides brasiliensis using bentonite particles as antigen (Bent-IF). The bentonite particles were coated with P. brasiliensis polysaccharide antigen and tested with sera from paracoccidioidomycosis patients (36 sera), normal blood donors (32 sera) and patients with non-mycotic diseases (29 sera). The titres given by the positive sera were compared with those of complement fixation (CF), immunodiffusion (ID) and immunofluorescent test using yeast forms of the fungus as antigen (conventional-IF). All normal blood donors' sera gave a negative Bent-IF, conventional-IF, ID and CF tests. All paracoccidioidomycosis sera were reactive in conventional-IF and gave concordant results in Bent-IF. There was no correlation between CF and Bent-IF titres. 27·6% of sera from patients with non-mycotic diseases gave weak titres in both IF-tests. The present data indicate that the Bent-IF is a sensitive and simple serodiagnostic technique comparable with the conventional P. brasiliensis antibody test. © 1983.
How to cite this document
Showing items related by title, author, creator and subject.
Toxoplasma gondii: Comparison of a rhoptry-ELISA with IFAT and MAT for antibody detection in sera of experimentally infected pigs Garcia, João Luis; Navarro, Italmar Teodorico; Vidotto, Odilon; Gennari, Solange Maria; Machado, Rosângela Zacarias ; Pereira, Ademir Benedito da Luz; Sinhorini, Idercio Luiz (Experimental Parasitology, 2006) [Artigo]Indirect ELISA and IFAT have been reported to be more sensitive and specific than agglutination tests. However, MAT is cheaper, easier than the others and does not need special equipment. The purpose of this study was to ...
Victor Canettieri, Antonio Carlos ; Kretchetoff, Fujiko Yamasiro; Koga Ito, Cristiane Yumi ; Moreira, Daniella ; Condino Fujarra, Fabio José; Unterkircher, Carmelinda Schmidt (Brazilian Oral Research, 2006) [Artigo]Several studies have been conducted in the last decades aiming to obtain an anti-canies vaccine, however some studies have demonstrated cross reactivity between Streptococcus mutans surface antigens and the human cardiac ...
Autoimmunity does not contribute to the highly prevalent glucose metabolism disturbances in a Japanese Brazilian population Hashimoto, Silvia M.; Hirai, A. T.; Kikuchi, M.; Cardoso, M. A. ; Tomita, N.; Chaim, R.; Wakisaka, K.; Gimeno, Suely G.A.; Matsumura, Luiza; Franco, Laercio J. et al. (Ethnicity and Disease, 2007) [Artigo]The Japanese Brazilian population has one of the highest prevalences of diabetes worldwide. Despite being non-obese according to standard definitions, their body fat distribution is typically central. We investigated whether ...