Development of SCAR marker linked to stem canker resistance gene in soybean
Carregando...
Data
2007-06-01
Autores
Gavioli, Eduardo Antonio
Di Mauro, Antonio Orlando [UNESP]
Centurion, Maria Aparecida Pessoa da Cruz [UNESP]
Di Mauro, Sônia Marli Zingaretti [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Stem canker caused by the fungus Diaporthe phaseolorum f. sp. meridionalis is a disease that limits soybean cultivation. Phenotypic evaluations aiming at disease resistance require labor-intensive processes, as for instance handling and transport of phytopathogens. The use of DNA markers in the selective procedures eases certain phases, besides being practical, safe and reliable. A RAPD fragment of 588pb was identified among bulks of resistant and susceptible plants in the cross BR92-15454 (R) x IAC-11 (S). Through co-segregation, the distance between the resistance locus and the fragment was estimated at 7.4 ± 2.1 cM, with a Lodmax. of 23.072 (first year) and at 6.0 ± 3.4 cM with a Lodmax. of 7.806 (second year). The fragment was converted into a SCAR marker and digested with enzyme Hinc II, which made the classification in homozygous resistant, heterozygous resistant and susceptible plants possible. This SCAR marker is suitable for use in the improvement program conducted in Jaboticabal.
Descrição
Palavras-chave
Assisted selection, Diaporthe phaseolorum f. sp. meridionalis, Glycine max, RAPD, Diaporthe phaseolorum, Fungi
Como citar
Crop Breeding and Applied Biotechnology, v. 7, n. 2, p. 133-140, 2007.