Taenia solium and Taenia saginata: Identification of sequence characterized amplified region (SCAR) markers
MetadataShow full item record
Cysticercosis is one of the most important zoonosis, not only because of the effects on animal health and its economic consequences, but also due to the serious danger it poses to humans. The two main parasites involved in the taeniasis-cysticercosis complex in Brazil are Taenia saginata and Taenia solium. Differentiating between these two parasites is important both for disease control and for epidemiological studies. The purpose of this work was to identify genetic markers that could be used to differentiate these parasites. Out of 120 oligonucleotide decamers tested in random amplified polymorphic DNA (RAPD) assays, 107 were shown to discriminate between the two species of Taenia. Twenty-one DNA fragments that were specific for each species of Taenia were chosen for DNA cloning and sequencing. Seven RAPD markers were converted into sequence characterized amplified region (SCAR) markers with two specific for T. saginata and five specific for T. solium as shown by agarose gel electrophoresis. These markers were developed as potential tools to differentiate T. solium from T. saginata in epidemiological studies. © 2007 Elsevier Inc. All rights reserved.
How to cite this document
Showing items related by title, author, creator and subject.
Paulan, Silvana de Cássia (Universidade Estadual Paulista (UNESP), 2015) [Tese de doutorado]Taenia saginata represents a threat to food security and public health in consequence of human infection by ingestion of contaminated undercooked meat. This zoonosis is worldwide distributed, affecting human, definitive ...
Aragão, Samuel Carvalho de ; Biondi, Germano Francisco ; Lima, Luis Gustavo Ferraz ; Nunes, Cáris Maroni (Revista Brasileira de Parasitologia Veterinária, 2010) [Artigo]A inspeção sanitária da carne bovina e suína tem sido a principal forma diagnóstica da cisticercose animal e da prevenção da teníase no Brasil. As aldeias indígenas Jaguapirú e Bororó estão localizadas próximo à área urbana ...
Inquérito epidemiológico sobre teníase em população do programa saúde da família no município de Uberaba, MG Esteves, Flavia Maria ; Silva-Vergara, Mario León; Carvalho, Ângela C.F. Banzatto de (Revista da Sociedade Brasileira de Medicina Tropical, 2005) [Artigo]Foi realizado inquérito para avaliar alguns aspectos epidemiológicos da teníase em 100.144 indivíduos do Programa Saúde da Família. Foram identificados 185 (0,2%) indivíduos com antecedentes de teníase. Destes, 112 (60,5%) ...