Cytotoxic effects of different concentrations of chlorhexidine
MetadataShow full item record
Purpose: To evaluate the cytotoxic effects of different concentrations of Chlorhexidine (Chx) to the odontoblast cell line MDPC-23. Methods: The odontoblast-like cells were seeded (30,000 cells/cm 2) in 60 wells of 24-well dishes and then incubated in contact with the following experimental and control solutions: Group 1: 0.0024% Chx; Group 2: 0.004% Chx; Group 3: 0.02% Chx; Group 4: Phosphate buffer saline solution (PBS, negative control); and Group 5: 0.06% H 2O 2 (positive control). Cell metabolic activity was measured by MTT assay and the cell morphology was analyzed by SEM. Results: The cytotoxic effects of Chx are dose-dependent. The reduction in the cell metabolism for Groups 1, 2, and 3 was 24.8%, 29.9% and 70.8%, respectively. No statistical difference was observed between the Groups 1 and 2 in which no significant cell morphology changes occurred. Consequently, it was concluded that 0.02% Chx solution presents high cytotoxicity to the odontoblast-like cells MDPC-23. On theother hand, 0.0024% and 0.004% Chx causes slight cytopathic effects to the cultured cells.
How to cite this document
Showing items related by title, author, creator and subject.
Balbani, Aracy Pereira Silveira; Stelzer, Lucilena Bardella ; Montovani, Jair Cortez (Revista Brasileira de Otorrinolaringologia, 2006) [Artigo]Aim: To evaluate the presence of preservatives, dyes, sweeteners and flavouring substances in 73 pharmaceutical preparations of 35 medicines for oral administration, according to drug labeling information about the excipients. ...
Viegas-Junior, Cláudio ; Danuello, Amanda ; Bolzani, Vanderlan da Silva ; Barreiro, Eliezer J.; Fraga, Carlos Alberto M. (Current Medicinal Chemistry, 2007) [Resenha]Molecular hybridization is a new concept in drug design and development based on the combination of pharmacophoric moieties of different bioactive substances to produce a new hyrid compound with improved affinity and ...
Farmacología en la tercera edad: Medicamentos de uso continuo y peligros de la interacción medicamentosa Haddad, Marcela Filié ; Takamiya, Aline Satie ; da Silva, Eulália Maria Martins ; Barbosa, Débora de Barros (Gerokomos, 2009) [Artigo]Introduction: It was observed a considerable growth of elderly people. They are who use more medicines. The physiological changes associated with the age advancing can make pharmacokinetic and pharmacodynamic alterations. ...