Doxazosin reduces cell proliferation and increases collagen fibers in rat prostatic lobes
MetadataShow full item record
We investigated the effects of doxazosin (Dox), an alpha-adrenoceptor antagonist used clinically for the treatment of benign prostatic hyperplasia (BPH), on the rat prostatic complex by assessing structural parameters, collagen fiber content, cell proliferation, and apoptosis. Adult Wistar rats were treated with Dox (25 mg/kg per day), and the ventral (VP), dorsolateral, and anterior prostate (AP) regions of the prostate complex were excised at 3, 7, and 30 days after treatment. At 24 h before being killed, the rats were injected once with 5-bromodeoxyuridine (BrdU; thymidine analog) to label mitotically active cells. The prostates were weighed and processed for histochemistry, morphometry-stereology, immunohistochemistry for BrdU, Western blotting for proliferating cell nuclear antigen (PCNA), and the TUNEL reaction for apoptosis. Dox-treated prostate lobes at day 3 presented increased weight, an enlarged ductal lumen, low cubical epithelial cells, reduced epithelial folds, and stretched smooth muscle cells. However, at day 30, the prostates exhibited a weight reduction of ∼20% and an increased area of collagen and reticular fibers in the stromal space. Dox also reduced epithelial cell proliferation and increased apoptosis in the three prostatic lobes. Western blotting for PCNA confirmed the reduction of cell proliferation by Dox, with the AP and VP being more affected than the dorsal prostate. Thus, Dox treatment alters epithelial cell behavior and prostatic tissue mechanical demand, inducing tissue remodeling in which collagen fibers assume a major role. © 2007 Springer-Verlag.
How to cite this document
Showing items related by title, author, creator and subject.
Taboga, S. R. ; De Campos Vidal, B. (Journal of Submicroscopic Cytology and Pathology, 2003) [Artigo]The present study focuses on establishing patterns of collagen fibers distribution in prostatic nodular hyperplasia and adenocarcinomas, in comparison with the normal tissue. Sections of prostatic transurethral resection ...
Chronic Ethanol Consumption Alters All-Trans-Retinoic Acid Concentration and Expression of Their Receptors on the Prostate: A Possible Link Between Alcoholism and Prostate Damage Fontanelli, Beatriz A.F. ; Chuffa, Luiz G.A. ; Teixeira, Giovana R. ; Amorim, João P.A. ; Mendes, Leonardo O. ; Pinheiro, Patricia F.F. ; Kurokawa, Cilmery Suemi ; Pereira, Sérgio ; Fávaro, Wagner J. ; Martins, Otávio A. et al. (Alcoholism: Clinical and Experimental Research, 2013) [Artigo]Background: Ethanol (EtOH) alters the all-trans-retinoic acid (ATRA) levels in some tissues. Retinol and ATRA are essential for cell proliferation, differentiation, and maintenance of prostate homeostasis. It has been ...
Age-related histopathological lesions in the Mongolian gerbil ventral prostate as a good model for studies of spontaneous hormone-related disorders Campos, Silvana Gisele Pegorin; Zanetoni, Cristiani; Scarano, Wellerson Rodrigo; Vilamaior, Patricia Simone Leite ; Taboga, Sebastiao Roberto (International Journal of Experimental Pathology, 2008) [Artigo]The Meriones unguiculatus (Mongolian) gerbil has demonstrated significant prostatic responses to hormonal treatments, and to drugs against human prostatic hyperplasia Spontaneous neoplasia develops in the older animals. ...