Publicação: Effects of zoledronic acid on odontoblast-like cells
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The aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5 × 104 cells/cm2) were seeded for 48 h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24 h, ZOL (1 or 5 μM) was added to the medium and maintained in contact with the cells for 24 h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability-MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five μM ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1 μM ZOL caused a significant increase in Col-I expression. Although 5 μM ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p < 0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. © 2012 Elsevier Ltd.
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Bisphosphonates, Cell culture, Cytotoxicity, alkaline phosphatase, bisphosphonic acid derivative, bone density conservation agent, collagen type 1, dentin sialophosphoprotein, imidazole derivative, phosphoprotein, protein synthesis inhibitor, scleroprotein, sialoglycoprotein, succinate dehydrogenase, zoledronic acid, biosynthesis, cell culture, cell survival, dose response, drug antagonism, drug effect, gene expression regulation, human, odontoblast, Alkaline Phosphatase, Bone Density Conservation Agents, Cell Survival, Cells, Cultured, Collagen Type I, Diphosphonates, Dose-Response Relationship, Drug, Extracellular Matrix Proteins, Gene Expression Regulation, Enzymologic, Humans, Imidazoles, Odontoblasts, Phosphoproteins, Protein Synthesis Inhibitors, Sialoglycoproteins, Succinate Dehydrogenase, Bovinae
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Archives of Oral Biology, v. 58, n. 5, p. 467-473, 2013.
