Análise comparativa da expressão protéica em glândulas pós-faríngeas de saúva-limão, Atta sexdens rubropilosa Forel, 1908 (Hymenoptera: Formicidae) submetidas à suplementação lipídica
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Data
2013-02-26
Autores
Decio, Pâmela [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
As glândulas pós-faríngeas estão presentes em todas as formigas e em algumas vespas solitárias. Localizam-se dorso-lateralmente na transição entre a faringe e o esôfago e sua morfologia é variável entre os diferentes grupos de Formicidae, podendo apresentarse na forma lobulada ou digitada. As funções atribuídas a essas glândulas são passíveis de discussão. São consideradas por diferentes autores como cecos gástricos, divertículo do trato digestório, como produtoras de feromônios ou de hidrocarbonetos cuticulares. Novas evidências tem dado suporte a função dessas glândulas na ingestão e retenção de lipídios. Com isto em vista, o presente trabalho teve como objetivo identificar as proteínas expressas nas glândulas pós-faríngeas de Atta sexdens rubropilosa buscando ampliar o conhecimento sobre uma possível função destas glândulas no metabolismo de lipídios por meio da comparação das proteínas diferencialmente expressas nas glândulas de formigas em três estágios alimentares: rainhas que não receberam nenhum tipo de tratamento alimentar e rainhas que receberam óleo de soja como suplementação lipídica, analisadas 4 e 120 horas após o tratamento. A utilização da abordagem proteômica envolveu técnicas de eletroforese bidimensional, estratégia shotgun e espectrometria de massas. Para a análise dos resultados obtidos foram usadas diferentes ferramentas de bioinformática para a identificação das proteínas e esclarecimento de suas funções. O algoritmo utilizado para pesquisa nos bancos de dados foi o MASCOT Ion Search versão 2.0 e os bancos de dados foram o Protein Data Bank, SwissProt e o GenBank. Foram identificadas 33 proteínas a partir do extrato de glândulas pós-faríngeas de rainhas Atta sexdens rubropilosa, envolvidas em diversas funções como metabolismo de lipídios, energético e de esteróides, bem como com os mecanismos de defesa, regulação metabólica, transdução de sinal e...
The post-pharyngeal glands are present in all ants and some solitary wasps. These glands are located laterally in the back of the head, between the pharynx and esophagus, and their morphology varies among different groups of Formicidae. They may present themselves as lobulated or typed. The functions assigned to these glands are open to discussion, and are considered by different authors as gastric caeca, diverticulum of the digestive tract or as producer of pheromones or cuticular hydrocarbons. New evidence has supported the function of these glands to be in the intake and retention of lipids. The present study aimed to identify the proteins present in the post-pharyngeal glands of Atta sexdens rubropilosa seeking to expand knowledge about a possible function of these glands in the metabolism of lipids by comparing the differentially expressed proteins in the glands of ants food in three stages: queens who did not receive any treatment, and queens who received soybean oil as lipid supplementation analyzed 4h and 120h after treatment. Using the proteomic approach involving two-dimensional electrophoresis techniques, strategy and shotgun mass spectrometry. To analyze the obtained results various bioinformatics tools were used for protein identification and clarification of their duties. The algorithm used to search the databases was the MASCOT Ion Search version 2.0 and the databases were the Protein Data Bank, GenBank and SwissProt. In the end, 38 identified proteins were extracted of the post-pharyngeal glands of Atta sexdens rubropilosa queens, involved in several functions such as metabolism of lipid, energy and steroids, as well as defense mechanisms, metabolic regulation, signal transduction, cytoskeleton restoration and membrane protein. The expression of proteins related to lipid... (Complete abstract click electronic access below)
The post-pharyngeal glands are present in all ants and some solitary wasps. These glands are located laterally in the back of the head, between the pharynx and esophagus, and their morphology varies among different groups of Formicidae. They may present themselves as lobulated or typed. The functions assigned to these glands are open to discussion, and are considered by different authors as gastric caeca, diverticulum of the digestive tract or as producer of pheromones or cuticular hydrocarbons. New evidence has supported the function of these glands to be in the intake and retention of lipids. The present study aimed to identify the proteins present in the post-pharyngeal glands of Atta sexdens rubropilosa seeking to expand knowledge about a possible function of these glands in the metabolism of lipids by comparing the differentially expressed proteins in the glands of ants food in three stages: queens who did not receive any treatment, and queens who received soybean oil as lipid supplementation analyzed 4h and 120h after treatment. Using the proteomic approach involving two-dimensional electrophoresis techniques, strategy and shotgun mass spectrometry. To analyze the obtained results various bioinformatics tools were used for protein identification and clarification of their duties. The algorithm used to search the databases was the MASCOT Ion Search version 2.0 and the databases were the Protein Data Bank, GenBank and SwissProt. In the end, 38 identified proteins were extracted of the post-pharyngeal glands of Atta sexdens rubropilosa queens, involved in several functions such as metabolism of lipid, energy and steroids, as well as defense mechanisms, metabolic regulation, signal transduction, cytoskeleton restoration and membrane protein. The expression of proteins related to lipid... (Complete abstract click electronic access below)
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Palavras-chave
Ants, Formiga, Formiga-cortadeira, Glandulas, Proteômica, Proteínas, Espectrometria de massa, Lipidios
Como citar
DECIO, Pâmela. Análise comparativa da expressão protéica em glândulas pós-faríngeas de saúva-limão, Atta sexdens rubropilosa Forel, 1908 (Hymenoptera: Formicidae) submetidas à suplementação lipídica. 2013. 55 f. Dissertação - (mestrado) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro, 2013.