Efeito do soro urêmico de cães com insuficiência renal sobre o metabolismo oxidativo e apoptose dos polimorfonucleares
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Data
2009-06-22
Autores
Barbosa, Tatiana de Sousa [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Embora a insuficiência renal ocorra com bastante freqüência na espécie canina, não se sabe se essa condição, a semelhança do que ocorre em humano, compromete o funcionamento dos poliformonucleares (PMN). O superóxido produzido pelo metabolismo oxidativo dos PMN exerce importante papel na resposta imune inata, destruído os patógenos fagocitados, entretanto, quando em excesso age de modo deletério promovendo a aceleração da apoptose. Testou-se a hipótese de que, a semelhança do que ocorrer em humanos, as toxinas presentes no soro de cães urêmicos alteram o metabolismo oxidativo e acelera a morte celular programada dos neutrófilos de cães normais. Para tal o sangue total e polimorfonucleares isolados de dez cães sadios foram incubados com soro urêmico. A produção de superóxido foi quantificada pelo teste de redução do tetrazólio nitroazul (NBT) e o índice apoptótico calculado pelo método morfométrico. A produção de superóxido gerada dos neutrófilos de sangue total tratados com soro urêmico apresentou significante redução (p < 0,05). Quando isolados e incubados com soro urêmico, apenas na metade das amostras os PMN apresentaram concomitantemente diminuição da produção de superóxido e aumento do índice apoptótico. Foi possível concluir que os componentes presentes no soro urêmico alteram ex vivo o metabolismo oxidativo e a apoptose dos PMN, fortalecendo a hipótese de que cães com de insuficiência renal têm sua imunidade inata comprometida.
Although kidney failure occurs frequently on canine species, it is unknown if this condition, being similar to what occurs in human beings, jeopardized the functioning of the polymorphonuclear (PMN). The superoxide produced by the oxidative metabolism of the PMN plays an important role in the immune inherent answer, having destroyed the pathogenic phagocytized. However, when in excess it acts in a deleterious way promoting the acceleration of apoptosis. It was tested by the hypothesis that, similar to what occurs in humans, the toxins present in the serum of uremic dogs alter the oxidative metabolism and accelerates the programmed cellular death of the neutrophis of normal dogs. For that, the total blood and polymorphonuclears isolated from ten healthy dogs were incubated with uremic serum. The production of superoxide was quantified by nitroblue tetrazolium reduction test (NBT) and the index apoptic was calculated by the morphometric method. The production of superoxide generated from the neutrophil of total blood treated with uremic serum presented significant reduction (P<0.05). When isolated and incubated with uremic serum, only on half of the sample the PMN presented concomitantly a reduction of production of superoxide and increase of apoptotic index. It was possible deduct that the components present in the uremic serum alter ex vivo the oxidative metabolism and the apoptosis of the PMN, consolidating the hypothesis that dogs having kidney failure have their inherent immunity jeopardized.
Although kidney failure occurs frequently on canine species, it is unknown if this condition, being similar to what occurs in human beings, jeopardized the functioning of the polymorphonuclear (PMN). The superoxide produced by the oxidative metabolism of the PMN plays an important role in the immune inherent answer, having destroyed the pathogenic phagocytized. However, when in excess it acts in a deleterious way promoting the acceleration of apoptosis. It was tested by the hypothesis that, similar to what occurs in humans, the toxins present in the serum of uremic dogs alter the oxidative metabolism and accelerates the programmed cellular death of the neutrophis of normal dogs. For that, the total blood and polymorphonuclears isolated from ten healthy dogs were incubated with uremic serum. The production of superoxide was quantified by nitroblue tetrazolium reduction test (NBT) and the index apoptic was calculated by the morphometric method. The production of superoxide generated from the neutrophil of total blood treated with uremic serum presented significant reduction (P<0.05). When isolated and incubated with uremic serum, only on half of the sample the PMN presented concomitantly a reduction of production of superoxide and increase of apoptotic index. It was possible deduct that the components present in the uremic serum alter ex vivo the oxidative metabolism and the apoptosis of the PMN, consolidating the hypothesis that dogs having kidney failure have their inherent immunity jeopardized.
Descrição
Palavras-chave
Neutrofilos, Imunossupressão, Apoptose, Superoxido, Uremia, Morte celular, Superóxidos, Neutrophils, Immunosuppression, Cell death, Apoptosis, Superoxides, Uremia
Como citar
BARBOSA, Tatiana de Sousa. Efeito do soro urêmico de cães com insuficiência renal sobre o metabolismo oxidativo e apoptose dos polimorfonucleares. 2009. 63 f. +. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia e Curso de Medicina Veterinária, 2009.