Perfil de citocinas no soro e na secreção cervical de mulheres com lesão intraepitelial de baixo grau, lesão intraepitelial de alto grau e carcinoma epidermóide invasor
Carregando...
Data
2011-01-28
Autores
Marcolino, Larissa Doddi [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
A infecção por Papilomavírus Humano (HPV) oncogênico é o fator de risco mais importante para desenvolvimento de lesões intraepiteliais bem como para o carcinoma epidermóide invasor. A resposta imune tem um papel importante na história natural da infecção pelo HPV na cérvice uterina estando envolvida na persistência viral e na progressão das lesões. As citocinas são moléculas reguladoras da transcrição do HPV, sendo que o padrão Th1 contribui para o desenvolvimento da imunidade celular contra a neoplasia e infecção pelo HPV, e está relacionado com clearance dessa infecção. O padrão Th2 está associado com a persistência da infecção viral e com a progressão das lesões. Avaliar a concentração de IL-2, IL-4, IL-6, IL-10, IL-12, IFN-g e TNF-a na secreção cervical e no soro de mulheres portadoras de lesão intraepitelial de baixo grau (LIEBG), lesão intraepitelial de alto grau (LIEAG) e carcinoma epidermóide invasor (CE). Foram incluídas no estudo 109 mulheres com diagnóstico histopatológico de LIEBG (n=16), LIEAG (n=40), CE (n=13) e 40 mulheres com suspeita de doença HPV induzida, mas sem alterações histopatológicas na biópsia do colo do útero (grupo controle), atendidas no Ambulatório de Colposcopia da Faculdade de Medicina de Botucatu (UNESP) e no Ambulatório de Ginecologia Preventiva do Hospital Amaral Carvalho, Jaú, SP. Durante o exame especular foi coletada a secreção cervical com cytobrush para determinação do perfil de citocinas. O sangue periférico das mulheres incluídas no estudo foi colhido por punção venosa, em volume de 10mL e os padrões de citocinas no soro e na secreção cervical foram avaliados por ensaio imunoenzimático (ELISA). A pesquisa de HPV nas biópsias foi realizada empregando-se a técnica da reação em cadeia da polimerase (PCR) utilizando os primers específicos (MY9/11 e GP5+/GP6+) e a genotipagem foi realizada...
Infection with oncogenic types of human papillomavirus (HPV) is the most important factor for development of intraepithelial lesions and invasive squamous cell carcinoma. The immune response has an important role in the natural history of HPV infection in uterine cervix is involved in viral persistence and progression of lesions. Cytokines are molecules that regulate the transcription of HPV; the Th1 cytokine profile contributes to the development of cellular immunity against HPV infection and neoplasia, and is related to clearance of infection. The Th2 cytokine profile is associated with persistence of viral infection and the progression of lesions. Objectve: To evaluate the concentration of IL-2, IL-4, IL-6, IL- 10, IL-12, IFN-g and TNF-a in cervical secretion and serum of women with low-grade squamous intraepithelial lesions (LSIL), high-grade intraepithelial lesion (HSIL) and invasive squamous cell carcinoma (EC). The study included 109 women with histological diagnosis of LSIL (n = 16), HSIL (n = 40), EC (n = 13) and 40 women with suspected of HPVinduced disease, but no pathological changes on cervix biopsy (control group) attending in the Colposcopy Clinic of the Botucatu Medical School (UNESP) and in the Preventive Gynecology, Hospital Amaral Carvalho, Jaú, SP. During speculum examination cervical secretion was collected with cytobrush to determining the profile of cytokines. The peripheral blood of women enrolled in the study was collected by venipuncture in 10 mL volume and patterns of cytokines in serum and cervical secretion were assessed by enzyme immunoassay (ELISA). The search for HPV in the fragments of the biopsies were performed using the technique of polymerase chain reaction (PCR) using specific primers (MY9/11 and GP5+/GP6+) and genotyping was performed using the technique of PCR primers specific and confirmed by the analysis of restriction fragment polymorphism... (Complete abstract click electronic access below)
Infection with oncogenic types of human papillomavirus (HPV) is the most important factor for development of intraepithelial lesions and invasive squamous cell carcinoma. The immune response has an important role in the natural history of HPV infection in uterine cervix is involved in viral persistence and progression of lesions. Cytokines are molecules that regulate the transcription of HPV; the Th1 cytokine profile contributes to the development of cellular immunity against HPV infection and neoplasia, and is related to clearance of infection. The Th2 cytokine profile is associated with persistence of viral infection and the progression of lesions. Objectve: To evaluate the concentration of IL-2, IL-4, IL-6, IL- 10, IL-12, IFN-g and TNF-a in cervical secretion and serum of women with low-grade squamous intraepithelial lesions (LSIL), high-grade intraepithelial lesion (HSIL) and invasive squamous cell carcinoma (EC). The study included 109 women with histological diagnosis of LSIL (n = 16), HSIL (n = 40), EC (n = 13) and 40 women with suspected of HPVinduced disease, but no pathological changes on cervix biopsy (control group) attending in the Colposcopy Clinic of the Botucatu Medical School (UNESP) and in the Preventive Gynecology, Hospital Amaral Carvalho, Jaú, SP. During speculum examination cervical secretion was collected with cytobrush to determining the profile of cytokines. The peripheral blood of women enrolled in the study was collected by venipuncture in 10 mL volume and patterns of cytokines in serum and cervical secretion were assessed by enzyme immunoassay (ELISA). The search for HPV in the fragments of the biopsies were performed using the technique of polymerase chain reaction (PCR) using specific primers (MY9/11 and GP5+/GP6+) and genotyping was performed using the technique of PCR primers specific and confirmed by the analysis of restriction fragment polymorphism... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Colo uterino - Câncer - Estudo de caso, HPV, Cytokines
Como citar
MARCOLINO, Larissa Doddi. Perfil de citocinas no soro e na secreção cervical de mulheres com lesão intraepitelial de baixo grau, lesão intraepitelial de alto grau e carcinoma epidermóide invasor. 2011. 43 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, 2011.