Influência das alterações físicas e químicas sobre a função plaquetária e a expressão da GPIIbIIa em concentrados de plaquetas caninos estocados
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Data
2011-03-17
Autores
Costa, Ciniro [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O concentrado de plaquetas é um hemocomponente importante para a transfusão de grandes quantidades de plaquetas. Durante a estocagem, as plaquetas sofrem lesões que devem ser analisadas por meio de testes laboratoriais. O objetivo do presente estudo foi avaliar as qualidades físicas, químicas, microbiológicas, estruturais e funcionais plaquetárias em concentrados de plaquetas caninos estocados durante 5 e 7 dias. Foram realizadas contagem de plaquetas, leucócitos e hemácias por unidade, mensuração do pH, glicose, avaliação do swirling, análise microbiológica, avaliação da agregação plaquetária e da expressão da GPIIbIIIa pela citometria de fluxo. As análises foram realizadas logo após a centrifugação, e no quinto (grupo I) e sétimo (grupo II) dias de estocagem dos concentrados de plaquetas obtidos de 22 cães hígidos. Todas as bolsas foram negativas para o isolamento de microorganismos. Houve diminuição significativa nas contagens de plaquetas e leucócitos, pH, glicose e swirling nos dois grupos. Ocorreu diminuição na resposta agregatória nos dois grupos, com o uso isolado e associado de ADP e epinefrina. Os anticorpos CD41 e CD61 apresentaram alta porcentagem de fluorescência em todos os momentos, e não houve variação significativa na média de intensidade de fluorescência das plaquetas durante a estocagem. Conclui-se que, mantida a esterilidade microbiológica, os concentrados de plaquetas caninas podem ser estocados por até 7 dias, no entanto, há uma maior perda da viabilidade plaquetária e qualidade dos concentrados quando comparados ao grupo armazenado durante 5 dias. Houve perda funcional das plaquetas durante a estocagem pela redução da agregação plaquetária. Os anticorpos CD41 e CD61 podem ser utilizados na avaliação da GPIIbIIIa de plaquetas caninas estocadas durante os períodos avaliados
Platelet concentrate is an important blood component for platelet transfusion. During storage, platelets undergo lesions that should be evaluated by laboratory assays. The aim of this study was to evaluate the physical, chemical, microbiological, structural and functional platelet qualities in canine platelet concentrate stored for 5 and 7 days. Platelet, leukocytes and erythrocytes concentrations per unit, pH and glucose measurements, swirling observation, microbiological analysis, assessment of platelet agregation and expression of GPIIbIIIa by flow cytometry were performed. Analyses were performed after centrifugation, and the fifth (group I) and seventh (group II) days of platelet concentrate storage obtained from 22 healthy dogs. All platelet concentrate bags were negative for microorganisms isolation. Platelet and leukocytes concentrations, pH, glucose, and swirling decreased significantly during storage, in both groups. A significant reduction in aggregatory response was detected in both groups, in the presence of ADP, epinephrine or both simultaneously. CD41 and CD61 antibodies showed high fluorescence at all times, and no significant variation in mean fluorescence intensity during storage. In conclusion, canine platelet concentrates should be stored for 5 days, however, can be stored for up to 7 days with greater loss of viability and quality of bags during this period. There was functional loss observed by the reduction of platelet aggregation during storage in both groups. CD41 and CD61 antibodies can be used to assess GPIIbIIIa in canine platelets stored during the evaluated period
Platelet concentrate is an important blood component for platelet transfusion. During storage, platelets undergo lesions that should be evaluated by laboratory assays. The aim of this study was to evaluate the physical, chemical, microbiological, structural and functional platelet qualities in canine platelet concentrate stored for 5 and 7 days. Platelet, leukocytes and erythrocytes concentrations per unit, pH and glucose measurements, swirling observation, microbiological analysis, assessment of platelet agregation and expression of GPIIbIIIa by flow cytometry were performed. Analyses were performed after centrifugation, and the fifth (group I) and seventh (group II) days of platelet concentrate storage obtained from 22 healthy dogs. All platelet concentrate bags were negative for microorganisms isolation. Platelet and leukocytes concentrations, pH, glucose, and swirling decreased significantly during storage, in both groups. A significant reduction in aggregatory response was detected in both groups, in the presence of ADP, epinephrine or both simultaneously. CD41 and CD61 antibodies showed high fluorescence at all times, and no significant variation in mean fluorescence intensity during storage. In conclusion, canine platelet concentrates should be stored for 5 days, however, can be stored for up to 7 days with greater loss of viability and quality of bags during this period. There was functional loss observed by the reduction of platelet aggregation during storage in both groups. CD41 and CD61 antibodies can be used to assess GPIIbIIIa in canine platelets stored during the evaluated period
Descrição
Palavras-chave
Citometria de fluxo, Cão, Plaquetas (Sangue), Quality control
Como citar
COSTA, Carla Cristina Machado Riani. Influência das alterações físicas e químicas sobre a função plaquetária e a expressão da GPIIbIIa em concentrados de plaquetas caninos estocados. 2011. 80 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia, 2011.