Deteccão de diferentes microorganismos na periodontite crônica, da glicoproteína EMMPRIN (CD-147) e sua correlação com a produção de MMP-2 e MMP-9
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Data
2012-03-23
Autores
Toledo, Felipe Almeida de [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A periodontite é uma doença infecciosa caracterizada pela secreção de uma variedade de mediadores inflamatórios que levam a destruição dos tecidos de suporte dental, incluindo o osso alveolar e possível perda dos dentes, em associação com a infecção por múltiplas espécies de bactérias gram-negativas. Estima-se que mais de 400 espécies microbianas colonizam o biofilme dental e algumas das espécies bucais relacionadas à doença periodontal apresentem-se no biofilme subgengival incluindo Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola. Entretanto, outros microrganismos podem também estar relacionados a patologia desta doença, como Filifactor alocis, Prevotella tannerae e Candida albicans. Estes microrganismos juntamente com seus subprodutos como as endotoxinas liberadas no meio extracelular levam ao estímulo da glicoproteina Indutora de Metaloproteinases (EMMPRIN-CD147) a qual estimula a liberação de metaloproteinases da matriz (MMPs) pelas células do hospedeiro, por exemplo, fibroblastos e células endoteliais levando assim a destruição tecidual. Com o objetivo de detectar novos patógenos relacionados à doença periodontal como F. alocis, P. tannerae, C. albicans e T. denticola e também avaliar a glicoproteína EMMPRIN (CD- 147) e sua correlação com MMP-2 e MMP-9 em amostras de fluido subgengival de pacientes com periodontite crônica, foram coletadas amostras de fluido subgengival de sítios sadios e doentes de 20 indivíduos com periodontite crônica antes do tratamento periodontal básico, e após um período de 60 dias do tratamento foi realizada nova coleta de sítios sadios e doentes. Os respectivos DNAs foram extraídos e trechos do gene 16S foram amplificados e posteriormente realizados PCR convencional para a análise microbiológica dos microrganismos em estudo. Para a...
Periodontitis is an infectious disease characterized by the secretion of a variety of inflammatory mediators that lead to destruction of tooth supporting tissues, including the possible loss of alveolar bone and teeth, in association with infection with multiple species of gram-negative bacteria. It is estimated that more than 400 species of microorganisms colonize the biofilm and some oral species related to periodontal disease is present in the subgingival including Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. However, other organisms may also be related to pathology of this disease, as Filifactor allocis, Prevotella tannerae and Candida albicans. These microorganisms along with subproducts such as endotoxins released into the extracellular lead to the stimulation of metalloproteinase inducer glycoprotein (EMMPRIN, CD-147), which stimulates the release of MMPs by host cells, like fibroblasts and endothelial cells, thus leading to tissue destruction . The objective of this study was to detect new pathogens relationed with periodontal disease such as F. allocis, P. tannerae, C. albicans, T. denticola and also the glycoprotein EMMPRIN (CD-147) and its correlation with MMP-2 and MMP-9 in subgingival fluid samples of patients with chronic periodontitis. Fluid were collected from healthy and disease subgingival sites of 20 healthy individuals with chronic periodontitis before basic periodontal treatment, and after a period of 60 days of treatment was performed new collection of healthy and diseased sites. Their DNAs were extracted and portions of the 16S gene were amplified and subsequently performed conventional PCR for the microbiological analysis of the microorganisms this study. For immunological analysis and quantification of EMMPRIN (CD-147), MMP-2 and MMP-9 was used... (Complete abstract click electronic access below)
Periodontitis is an infectious disease characterized by the secretion of a variety of inflammatory mediators that lead to destruction of tooth supporting tissues, including the possible loss of alveolar bone and teeth, in association with infection with multiple species of gram-negative bacteria. It is estimated that more than 400 species of microorganisms colonize the biofilm and some oral species related to periodontal disease is present in the subgingival including Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. However, other organisms may also be related to pathology of this disease, as Filifactor allocis, Prevotella tannerae and Candida albicans. These microorganisms along with subproducts such as endotoxins released into the extracellular lead to the stimulation of metalloproteinase inducer glycoprotein (EMMPRIN, CD-147), which stimulates the release of MMPs by host cells, like fibroblasts and endothelial cells, thus leading to tissue destruction . The objective of this study was to detect new pathogens relationed with periodontal disease such as F. allocis, P. tannerae, C. albicans, T. denticola and also the glycoprotein EMMPRIN (CD-147) and its correlation with MMP-2 and MMP-9 in subgingival fluid samples of patients with chronic periodontitis. Fluid were collected from healthy and disease subgingival sites of 20 healthy individuals with chronic periodontitis before basic periodontal treatment, and after a period of 60 days of treatment was performed new collection of healthy and diseased sites. Their DNAs were extracted and portions of the 16S gene were amplified and subsequently performed conventional PCR for the microbiological analysis of the microorganisms this study. For immunological analysis and quantification of EMMPRIN (CD-147), MMP-2 and MMP-9 was used... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Reação em cadeia de polimerase, Candida albicans, Periodontite
Como citar
TOLEDO, Felipe Almeida de. Deteccão de diferentes microorganismos na periodontite crônica, da glicoproteína EMMPRIN (CD-147) e sua correlação com a produção de MMP-2 e MMP-9. 2012. 97 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araraquara, 2012.