Efeito da concentração espermática e da metodologia de inseminação artificial com sêmen equino congelado
Carregando...
Data
2010-01-15
Autores
Avanzi, Bruno Ribeiro [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
A utilização de sêmen criopreservado na reprodução de eqüinos depara-se com obstáculos como: variabilidade entre garanhões e ejaculados ao processo de criopreservação, taxas de prenhez inferiores ao sêmen fresco e refrigerado, manejo mais trabalhoso e oneroso das éguas e ausência de padrões de qualidade na distribuição e comercialização do sêmen. Neste trabalho foram realizados dois experimentos, no qual primeiramente, avaliou-se o efeito de diferentes concentrações espermáticas em palhetas de 0,5 mL (100, 200 e 400 x 106 de espermatozóides totais/mL) sobre algumas variáveis de qualidade seminal pós-descongelação como: parâmetros de cinética espermática através de análise computadorizada, integridade de membrana plasmática e resistência à peroxidação lipídica. Posteriormente, realizou-se um estudo comparativo entre a inseminação pós-ovulação e a inseminação em tempo pré-determinado, avaliando-se os resultados de concepção e acúmulo de fluido intra-uterino pós-inseminação. Ao empregar a metodologia de inseminação pós-ovulação, realizou-se uma única inseminação dentro do intervalo de até 6 horas pós-ovulação com 800x106 de espermatozóides totais e para o método programado, realizaram-se duas inseminações após 24 e 40 horas pós-indução da ovulação, com 400x106 de espermatozóides totais cada. Foi observado efeito significativo da concentração espermática (p<0,05) sobre algumas variáveis de cinética espermática como: motilidade total, motilidade progressiva, velocidade ao longo de uma trajetória média, velocidade progressiva, velocidade curvilinear, freqüência de batimento flagelar e espermatozóides rápidos, com superioridade para a criopreservação em concentrações mais baixas. No entanto, para as variáveis de integridade de membrana plasmática e resistência à peroxidação lipídica não foram...
The cryopreserved semen application in equine reproduction appears with obstacles as variability between stallions and ejaculates at the cryopreservation process, lower pregnancy rates compared to the fresh and cooled semen, harder and onerous mares’ management and absence of quality pattern in distribution and semen commercialization. In this study were realized two experiments which one, firstly, was evaluated the effect of different sperm concentrations in straws of 0.5 mL (100, 200 and 400x106 total sperm/mL) over some variables of seminal quality after thaw as: parameters from sperm kinetics by computer analysis, plasmatic membrane integrity and resistance to lipid peroxidation. Subsequently, it was realized a comparative study between the insemination after ovulation and the insemination in fixed-time, evaluating the results of conception and intrauterine fluid collection after insemination. When the methodology of insemination after ovulation was employed, it was realized a single insemination during the interval of 6 hours after ovulation with 800x106 total spermatozoa and for the programmed method, two inseminations with 400x106 doses of total spermatozoa each one, were done after 24 and 40 hours after induction of ovulation. It was observed significant effect from sperm concentration (p<0,05) over some variables of sperm kinetics like: total motility, progressive motility, velocity in the average path velocity, straight line velocity, curvilinear velocity, beat cross frequency and rapid spermatozoa, with superiority for cryopreservation in lower concentrations. However, for the variables of plasmatic membrane integrity and resistance to lipid peroxidation were not observed significant effects. In respect to the insemination methods, it was not observed significative effect (p<0.05) over the pregnancy rates between one insemination after ovulation and two inseminations... (Complete abstract click electronic access below)
The cryopreserved semen application in equine reproduction appears with obstacles as variability between stallions and ejaculates at the cryopreservation process, lower pregnancy rates compared to the fresh and cooled semen, harder and onerous mares’ management and absence of quality pattern in distribution and semen commercialization. In this study were realized two experiments which one, firstly, was evaluated the effect of different sperm concentrations in straws of 0.5 mL (100, 200 and 400x106 total sperm/mL) over some variables of seminal quality after thaw as: parameters from sperm kinetics by computer analysis, plasmatic membrane integrity and resistance to lipid peroxidation. Subsequently, it was realized a comparative study between the insemination after ovulation and the insemination in fixed-time, evaluating the results of conception and intrauterine fluid collection after insemination. When the methodology of insemination after ovulation was employed, it was realized a single insemination during the interval of 6 hours after ovulation with 800x106 total spermatozoa and for the programmed method, two inseminations with 400x106 doses of total spermatozoa each one, were done after 24 and 40 hours after induction of ovulation. It was observed significant effect from sperm concentration (p<0,05) over some variables of sperm kinetics like: total motility, progressive motility, velocity in the average path velocity, straight line velocity, curvilinear velocity, beat cross frequency and rapid spermatozoa, with superiority for cryopreservation in lower concentrations. However, for the variables of plasmatic membrane integrity and resistance to lipid peroxidation were not observed significant effects. In respect to the insemination methods, it was not observed significative effect (p<0.05) over the pregnancy rates between one insemination after ovulation and two inseminations... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Equino - Reprodução, Inseminação artificila, Equine, Semen, Stattion, Artificial innsemination, Concentration
Como citar
AVANZI, Bruno Ribeiro. Efeito da concentração espermática e da metodologia de inseminação artificial com sêmen equino congelado. 2010. 66 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, 2010.