Efeito do ultra-som terapêutico sobre o crescimento ósseo das epífises, distal do fêmur e proximal da tíbia em Rattus novegicus
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Data
2009-10-02
Autores
Barreto, Alfredo Alcantara [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O propósito deste estudo é determinar os efeitos do ultra-som terapêutico em baixa potência sobre a disco epifisial e o crescimento ósseo distal do fêmur e proximal da tíbia, em ratos jovens. Ratus Norvegicus com quatro semanas de vida, machos, totalizando 105 animais, foram submetidos à terapêutica de ultra-som (1,0 MHz, potência de 0,4 watt/cm2, cabeçote fixo, pulso contínuo, 10 minutos de duração, três ou quatro vezes por semana), na face medial do joelho direito. Um grupo de animais foi submetido a 30 aplicações de ultra-som, até a 13ª semana de vida e, outro a 45 aplicações, até a 18ª semana de vida. Os animais foram acompanhados até os seis meses. Foram estudadas as medidas do comprimento do fêmur e da tíbia e da largura das epífises distal do fêmur e proximal da tíbia e, a morfometria da altura do disco epifisial, como um todo e, de suas zonas histológicas. Os dados foram submetidos à análise de variância – fatorial inteiramente aleatorizado no nível de significância de 5%. Não foram encontradas diferenças significativas no crescimento ósseo longitudinal ou das larguras das epífises quando comparados lados direito e esquerdo nos grupos tratados (G1 e G2) e lado direito dos grupos tratados em relação ao respectivo controle (G3 e G4). Não houve diferença quando comparados os grupos tratados com 30 aplicações e 45 aplicações de ultra-som. A morfometria mostrou diferenças, somente na tíbia, em que tratado foi maior que controle, tanto para 30 quanto para 45 aplicações de ultra-som. Tratado foi maior do que controle, no disco epifisial, nos três momentos estudados; na zona de proliferação, somente na 13ª semana; e, na zona hipertrófica na 18ª e 26ª semanas. O ultra-som terapêutico, nas condições deste trabalho, não provocou alterações do comprimento...
The purpose of this study was to determining the effects of lowpower ultrasound therapy on the epiphyseal plate and the bone growth of the distal femur and the proximal tibia in young rats. One hundred five of four-week-old Rattus norvegicus males received ultrasound treatment of continuous therapeutic dose (1.0 MHz frequency, 0.4 watt/cm2, fixed probe, 10 minutes three or four times a week) on the medial face of the right knee. One group of animals underwent 30 ultrasound applications until the age of 13 weeks, and the other received 45 applications until the age of 18 weeks. The animals were followed up until they were six month old (26 weeks). Assessment included the measurement of the length of the femur and the tibia, the width of the femoral distal epiphysis and the proximal tibial epiphysis, and the morphometry of the reserve plus proliferative (Proliferative) and hypertrophic plus calcified hypertrophic (Hipertrophic) zones, as well as width of the epiphyseal plate. Totallyrandomized factorial variance analysis of the data was performed with significance level set at 5%. No significant differences were observed in longitudinal bone growth or epiphyseal width when right and left sides were compared in treated groups (G1 and G2) or between the right side of the treated group and untreated groups (G3 and G4). There was no difference between the group receiving 30 ultrasound applications and that receiving 45 applications. Morphometry revealed that, in comparison with their controls, the groups treated with both 30 and 45 ultrasound applications showed differences only in the tibia. The epiphyseal plate width was larger at all three study time points. The Proliferative zone was increased at week 13 only, and the Hypertrophic zone at weeks 13 and 26. In this study, ultrasound therapy caused no change in the longitudinal growth... (Complete abstract click electronic access below)
The purpose of this study was to determining the effects of lowpower ultrasound therapy on the epiphyseal plate and the bone growth of the distal femur and the proximal tibia in young rats. One hundred five of four-week-old Rattus norvegicus males received ultrasound treatment of continuous therapeutic dose (1.0 MHz frequency, 0.4 watt/cm2, fixed probe, 10 minutes three or four times a week) on the medial face of the right knee. One group of animals underwent 30 ultrasound applications until the age of 13 weeks, and the other received 45 applications until the age of 18 weeks. The animals were followed up until they were six month old (26 weeks). Assessment included the measurement of the length of the femur and the tibia, the width of the femoral distal epiphysis and the proximal tibial epiphysis, and the morphometry of the reserve plus proliferative (Proliferative) and hypertrophic plus calcified hypertrophic (Hipertrophic) zones, as well as width of the epiphyseal plate. Totallyrandomized factorial variance analysis of the data was performed with significance level set at 5%. No significant differences were observed in longitudinal bone growth or epiphyseal width when right and left sides were compared in treated groups (G1 and G2) or between the right side of the treated group and untreated groups (G3 and G4). There was no difference between the group receiving 30 ultrasound applications and that receiving 45 applications. Morphometry revealed that, in comparison with their controls, the groups treated with both 30 and 45 ultrasound applications showed differences only in the tibia. The epiphyseal plate width was larger at all three study time points. The Proliferative zone was increased at week 13 only, and the Hypertrophic zone at weeks 13 and 26. In this study, ultrasound therapy caused no change in the longitudinal growth... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Ossos - Crescimento - Estudos experimentais, Ultrasound therapy, Rats, Epiphyses
Como citar
BARRETO, Alfredo Alcantara. Efeito do ultra-som terapêutico sobre o crescimento ósseo das epífises, distal do fêmur e proximal da tíbia em Rattus novegicus. 2009. 147 f. Tese (doutrado) - Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, 2009.