Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose

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dc.contributor.authorGoulart, Antonio José [UNESP]
dc.contributor.authorFrancisco dos Santos, Andréa [UNESP]
dc.contributor.authorTavano, Olga Luisa
dc.contributor.authorCésar Vinueza, Julio [UNESP]
dc.contributor.authorContiero, Jonas [UNESP]
dc.contributor.authorMonti, Rubens [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Triângulo Mineiro (UFTM)
dc.date.accessioned2014-05-27T11:29:47Z
dc.date.available2014-05-27T11:29:47Z
dc.date.issued2013-06-27
dc.description.abstractInvertase from Saccharomyces cerevisiae was immobilized on agarose beads, activated with various groups (glyoxyl, MANAE or glutaraldehyde), and on some commercial epoxy supports (Eupergit and Sepabeads). Very active and stable invertase derivatives were produced by the adsorption of the enzyme on MANAE-agarose, MANAE-agarose treated with glutaraldhyde and glutaraldehyde-agarose supports. At pH 5.0, these derivatives retained full activity after 24h at 40°C and 50 °C. When assayed at 40°C and 50°C, with the pH adjusted to 7.0, the invertase-MANAE-agarose derivative treated with glutaraldehyde retained 80% of the initial activity. Recovered activities of the derivatives produced with MANAE, MANAE treated with glutaraldehyde and glutaraldehyde alone were 73.5%, 44.4% and 36.8%, respectively. These three preparations were successfully employed to produce glucose and fructose in 3 cycles of sucrose hydrolysis.en
dc.description.affiliationDepartment of Food and Nutrition Universidade Estadual Paulista (UNESP), Araraquara, SP
dc.description.affiliationDepartment of Biochemistry and Technological Chemistry Instituto de Química Universidade Estadual Paulista (UNESP), Araraquara, SP
dc.description.affiliationUniversidade Federal do Triangulo Mineiro, Uberaba, MG
dc.description.affiliationDepartment of Biochemistry and Microbiology Instituto de Biociências Universidade Estadual Paulista (UNESP), Rio Claro, SP
dc.description.affiliationUnespDepartment of Food and Nutrition Universidade Estadual Paulista (UNESP), Araraquara, SP
dc.description.affiliationUnespDepartment of Biochemistry and Technological Chemistry Instituto de Química Universidade Estadual Paulista (UNESP), Araraquara, SP
dc.description.affiliationUnespDepartment of Biochemistry and Microbiology Instituto de Biociências Universidade Estadual Paulista (UNESP), Rio Claro, SP
dc.format.extent169-175
dc.identifierhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/2570
dc.identifier.citationRevista de Ciencias Farmaceuticas Basica e Aplicada, v. 34, n. 2, p. 169-175, 2013.
dc.identifier.file2-s2.0-84879254913.pdf
dc.identifier.issn1808-4532
dc.identifier.lattes9859154979447005
dc.identifier.scopus2-s2.0-84879254913
dc.identifier.urihttp://hdl.handle.net/11449/75722
dc.language.isoeng
dc.language.isopor
dc.relation.ispartofRevista de Ciências Farmacêuticas Básica e Aplicada
dc.relation.ispartofsjr0,131
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectGlucose and fructose production
dc.subjectInvertase
dc.subjectInvertase immobilization
dc.subjectSaccharomyces cerevisiae
dc.subjectagarose
dc.subjectbeta fructofuranosidase
dc.subjectepoxy resin
dc.subjectfructose
dc.subjectglucose
dc.subjectglutaraldehyde
dc.subjectsucrose
dc.subjectadsorption
dc.subjectbiotechnological production
dc.subjectenzyme activity
dc.subjectenzyme assay
dc.subjectenzyme immobilization
dc.subjectenzyme stability
dc.subjecthydrolysis
dc.subjectkinetics
dc.subjectnonhuman
dc.subjectpH
dc.subjecttemperature
dc.subjectthermostability
dc.titleProdução de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarosept
dc.title.alternativeGlucose and fructose production by Saccharomyces cerevisiae invertase immobilized on MANAE-agarose supporten
dc.typeArtigo
dcterms.licensehttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/about
unesp.author.lattes9859154979447005
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
unesp.departmentAlimentos e Nutrição - FCFpt

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