Sequence-Based Platforms for Discovering Biomarkers in Liquid Biopsy of Non-Small-Cell Lung Cancer

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dc.contributor.authorBrockley, Liam J.
dc.contributor.authorSouza, Vanessa G. P. [UNESP]
dc.contributor.authorForder, Aisling
dc.contributor.authorPewarchuk, Michelle E.
dc.contributor.authorErkan, Melis
dc.contributor.authorTelkar, Nikita
dc.contributor.authorBenard, Katya
dc.contributor.authorTrejo, Jessica
dc.contributor.authorStewart, Matt D.
dc.contributor.authorStewart, Greg L.
dc.contributor.authorReis, Patricia P. [UNESP]
dc.contributor.authorLam, Wan L.
dc.contributor.authorMartinez, Victor D.
dc.contributor.institutionBritish Columbia Cancer Research Institute
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionIWK Health Centre
dc.contributor.institutionDalhousie University
dc.contributor.institutionBeatrice Hunter Cancer Research Institute
dc.contributor.institutionBritish Columbia Children’s Hospital Research Institute
dc.date.accessioned2023-07-29T13:52:52Z
dc.date.available2023-07-29T13:52:52Z
dc.date.issued2023-04-01
dc.description.abstractLung cancer detection and monitoring are hampered by a lack of sensitive biomarkers, which results in diagnosis at late stages and difficulty in tracking response to treatment. Recent developments have established liquid biopsies as promising non-invasive methods for detecting biomarkers in lung cancer patients. With concurrent advances in high-throughput sequencing technologies and bioinformatics tools, new approaches for biomarker discovery have emerged. In this article, we survey established and emerging biomarker discovery methods using nucleic acid materials derived from bodily fluids in the context of lung cancer. We introduce nucleic acid biomarkers extracted from liquid biopsies and outline biological sources and methods of isolation. We discuss next-generation sequencing (NGS) platforms commonly used to identify novel biomarkers and describe how these have been applied to liquid biopsy. We highlight emerging biomarker discovery methods, including applications of long-read sequencing, fragmentomics, whole-genome amplification methods for single-cell analysis, and whole-genome methylation assays. Finally, we discuss advanced bioinformatics tools, describing methods for processing NGS data, as well as recently developed software tailored for liquid biopsy biomarker detection, which holds promise for early diagnosis of lung cancer.en
dc.description.affiliationBritish Columbia Cancer Research Institute
dc.description.affiliationMolecular Oncology Laboratory Experimental Research Unit School of Medicine São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Pathology and Laboratory Medicine IWK Health Centre
dc.description.affiliationDepartment of Pathology Faculty of Medicine Dalhousie University
dc.description.affiliationBeatrice Hunter Cancer Research Institute
dc.description.affiliationBritish Columbia Children’s Hospital Research Institute
dc.description.affiliationDepartment of Surgery and Orthopedics Faculty of Medicine São Paulo State University (UNESP), SP
dc.description.affiliationUnespMolecular Oncology Laboratory Experimental Research Unit School of Medicine São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Surgery and Orthopedics Faculty of Medicine São Paulo State University (UNESP), SP
dc.description.sponsorshipUniversity of British Columbia
dc.description.sponsorshipBC Cancer Foundation
dc.description.sponsorshipCanadian Institutes of Health Research
dc.description.sponsorshipIdCanadian Institutes of Health Research: FRN-143345
dc.description.sponsorshipIdCanadian Institutes of Health Research: FRN-183775
dc.identifierhttp://dx.doi.org/10.3390/cancers15082275
dc.identifier.citationCancers, v. 15, n. 8, 2023.
dc.identifier.doi10.3390/cancers15082275
dc.identifier.issn2072-6694
dc.identifier.scopus2-s2.0-85153973325
dc.identifier.urihttp://hdl.handle.net/11449/248754
dc.language.isoeng
dc.relation.ispartofCancers
dc.sourceScopus
dc.subjectbioinformatics
dc.subjectCTCs
dc.subjectctDNA
dc.subjecthigh throughput
dc.subjectliquid biopsy
dc.subjectlung cancer
dc.subjectNGS
dc.titleSequence-Based Platforms for Discovering Biomarkers in Liquid Biopsy of Non-Small-Cell Lung Canceren
dc.typeResenha
unesp.author.orcid0000-0002-1561-1651[2]
unesp.author.orcid0000-0002-3520-2353[6]
unesp.author.orcid0000-0003-1308-4410[8]
unesp.author.orcid0000-0003-3775-3797[11]

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