Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats

dc.contributor.authorEsteves, Jonatas Caldeira [UNESP]
dc.contributor.authorAranega, Alessandra Marcondes [UNESP]
dc.contributor.authorBorrasca, Albanir Gabriel [UNESP]
dc.contributor.authorRuiz de Souza Fattah, Cristiane Mara [UNESP]
dc.contributor.authorGarcia-Junior, Idelmo Rangel [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2013-09-30T18:29:34Z
dc.date.accessioned2014-05-20T13:43:20Z
dc.date.available2013-09-30T18:29:34Z
dc.date.available2014-05-20T13:43:20Z
dc.date.issued2008-09-01
dc.description.abstractFrom a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group.en
dc.description.affiliationState Univ São Paulo, Dent Sch Aracatuba, Dept Surg & Integrate Clin, Aracatuba, SP, Brazil
dc.description.affiliationUnespState Univ São Paulo, Dent Sch Aracatuba, Dept Surg & Integrate Clin, Aracatuba, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 04/13990-0
dc.format.extent316-320
dc.identifierhttp://dx.doi.org/10.1590/S1678-77572008000500003
dc.identifier.citationJournal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 16, n. 5, p. 316-320, 2008.
dc.identifier.fileS1678-77572008000500003-en.pdf
dc.identifier.issn1678-7757
dc.identifier.lattes6636749858940359
dc.identifier.scieloS1678-77572008000500003
dc.identifier.urihttp://hdl.handle.net/11449/15103
dc.identifier.wosWOS:000266688400003
dc.language.isoeng
dc.publisherUniversidade de São Paulo (USP), Faculdade de Odontologia de Bauru
dc.relation.ispartofJournal of Applied Oral Science
dc.relation.ispartofjcr1.709
dc.relation.ispartofsjr0,645
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectBone transplantationen
dc.subjectDiabetes mellitusen
dc.subjectWound healingen
dc.titleRepair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic ratsen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/jaos/paboutj.htm
dcterms.rightsHolderUniv São Paulo Fac Odontologia Bauru
unesp.author.lattes6636749858940359[2]
unesp.author.orcid0000-0001-5856-7972[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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