Partial urethral obstruction of rabbit urinary bladder: stereological evidence that the increase in muscle content is mostly driven by changes in number, rather than size, of smooth muscle cells

Página do item simplificado
dc.contributor.authorSasahara, Tais H. de Castro
dc.contributor.authorMayhew, Terry M.
dc.contributor.authorRahal, Sheila Canevese [UNESP]
dc.contributor.authorFioretto, Emerson T.
dc.contributor.authorBalieiro, Julio C. C.
dc.contributor.authorRibeiro, Antonio A. C. M.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Nottingham
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:21:06Z
dc.date.available2014-05-20T15:21:06Z
dc.date.issued2007-04-01
dc.description.abstractThe effects of partial urethral obstruction on the detrusor muscle of rabbit urinary bladder were investigated using stereological sampling and estimation tools. Twelve female Norfolk rabbits (2.5-3.0 kg body weight) were divided into four groups: 3, 7 and 12 weeks after surgical intervention to produce a standard partial obstruction and unobstructed controls. Following removal, bladder axes (craniocaudal, dorsoventral and laterolateral) and organ weights were recorded. Bladders were prepared for light microscopy by multistage random sampling procedures. Stereological methods were used to estimate the volume of muscle and the packing density and total number of myocyte nuclei in each bladder. We also estimated mean myocyte volume and the mean cross-sectional area and length of myocytes. Group comparisons were made by one-way analysis of variance. Changes in bladder axes were mainly laterolateral and craniocaudal. Mean bladder weight increased roughly six-fold by 3 weeks and 17-fold by 12 weeks and was accompanied, on average, by 12- and 33-fold increases in total muscle volume. These variables did not differ at 3 and 7 weeks post-obstruction. Increases in muscle content were not accompanied by changes in packing densities but were associated with increases in the total numbers of myocyte nuclei (13-fold by 3 weeks, 28-fold by 12 weeks). Mean myocyte volume did not vary significantly between groups but cells in obstructed groups were shorter and wider. These findings support the notion that partial outflow obstruction leads to an increase in the number, but not mean volume, of myocytes. If due solely to myocyte mitosis, the total of 43 x 10(8) cells found at 12 weeks could be generated by the original complement of 15 x 10(7) cells if an average of only 2.1 x 10(6) new cells was produced every hour. In reality, even this modest proliferation rate is unlikely to be achieved because myocyte proliferation rates are very low and it is possible that new myocytes can arise by differentiation of mesenchymal or other precursor cells.en
dc.description.affiliationUniv São Paulo, Dept Cirurgia, Fac Med Vet & Zootecn, Lab Stereol & Chem Anat,Coll Vet Med, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniv Nottingham, Queens Med Ctr, Ctr Integrated Syst Biol & Med, Nottingham NG7 2RD, England
dc.description.affiliationSão Paulo State Univ, Dept Vet Surg & Anaesthesiol, Vet Med & Zootech Coll, Botucatu, SP, Brazil
dc.description.affiliationUniv São Paulo, Coll Anim Sci & Food Engn, Dept Basic Sci, Pirassununga, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Vet Surg & Anaesthesiol, Vet Med & Zootech Coll, Botucatu, SP, Brazil
dc.format.extent449-459
dc.identifierhttp://dx.doi.org/10.1111/j.1469-7580.2007.00702.x
dc.identifier.citationJournal of Anatomy. Oxford: Blackwell Publishing, v. 210, n. 4, p. 449-459, 2007.
dc.identifier.doi10.1111/j.1469-7580.2007.00702.x
dc.identifier.fileWOS000245694100008.pdf
dc.identifier.issn0021-8782
dc.identifier.lattes1497433265390194
dc.identifier.orcid0000-0002-9211-4093
dc.identifier.urihttp://hdl.handle.net/11449/32289
dc.identifier.wosWOS:000245694100008
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofJournal of Anatomy
dc.relation.ispartofjcr2.479
dc.relation.ispartofsjr1,078
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectbladderpt
dc.subjectobstructionpt
dc.subjectrabbitpt
dc.subjectsmooth muscle cellspt
dc.subjectstereologypt
dc.titlePartial urethral obstruction of rabbit urinary bladder: stereological evidence that the increase in muscle content is mostly driven by changes in number, rather than size, of smooth muscle cellsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Publishing
unesp.author.lattes1497433265390194
unesp.author.orcid0000-0002-9211-4093[3]
unesp.author.orcid0000-0003-0540-1850[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000245694100008.pdf
Tamanho:
206.49 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Cirurgia e Anestesiologia Veterinária - FMVZ