Genetic characterization of Toxoplasma gondii from Brazilian wildlife revealed abundant new genotypes

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dc.contributor.authorVitaliano, S. N.
dc.contributor.authorSoares, H. S.
dc.contributor.authorMinervino, A. H.H.
dc.contributor.authorSantos, A. L.Q.
dc.contributor.authorWerther, K.
dc.contributor.authorMarvulo, M. F.V.
dc.contributor.authorSiqueira, D. B.
dc.contributor.authorPena, H. F.J.
dc.contributor.authorSoares, R. M.
dc.contributor.authorSu, C.
dc.contributor.authorGennari, S. M.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionCurso de Medicina Veterinária das Faculdades Metropolitanas Unidas (UniFMU)
dc.contributor.institutionInstituto de Biodiversidade e Floresta, Universidade Federal do Oeste do Pará
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionInstituto Brasileiro para Medicina da Conservação
dc.contributor.institutionParque Estadual Dois Irmãos
dc.date.accessioned2022-04-29T08:44:53Z
dc.date.available2022-04-29T08:44:53Z
dc.date.issued2014-10-01
dc.description.abstractThis study aimed to isolate and genotype T.gondii from Brazilian wildlife. For this purpose, 226 samples were submitted to mice bioassay and screened by PCR based on 18S rRNA sequences. A total of 15 T.gondii isolates were obtained, including samples from four armadillos (three Dasypus novemcinctus, one Euphractus sexcinctus), three collared anteaters (Tamandua tetradactyla), three whited-lipped peccaries (Tayassu pecari), one spotted paca (Cuniculus paca), one oncilla (Leopardus tigrinus), one hoary fox (Pseudalopex vetulus), one lineated woodpecker (Dryocopus lineatus) and one maned wolf (Chrysocyon brachyurus). DNA from the isolates, originated from mice bioassay, and from the tissues of the wild animal, designated as primary samples, were genotyped by PCR-restriction fragment length polymorphism (PCR/RFLP), using 12 genetic markers (SAG1, SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L258, PK1, CS3 and Apico). A total of 17 genotypes were identified, with 13 identified for the first time and four already reported in published literature. Results herein obtained corroborate previous studies in Brazil, confirming high diversity and revealing unique genotypes in this region. Given most of genotypes here identified are different from previous studies in domestic animals, future studies on T.gondii from wildlife is of interest to understand population genetics and structure of this parasite.en
dc.description.affiliationDepartamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Doutor Orlando Marques de Paiva 87, Cidade Univ.
dc.description.affiliationCurso de Medicina Veterinária das Faculdades Metropolitanas Unidas (UniFMU), Rua Ministro Nelson Hungria, 541
dc.description.affiliationInstituto de Biodiversidade e Floresta, Universidade Federal do Oeste do Pará, Rua Vera Paz
dc.description.affiliationFaculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Avenida Pará, 1720, Campus Umuarama
dc.description.affiliationDepartamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Prof. Paulo Donato Castellane, s/n, Campus de Jaboticabal
dc.description.affiliationInstituto Brasileiro para Medicina da Conservação
dc.description.affiliationParque Estadual Dois Irmãos
dc.description.affiliationDepartment of Microbiology, The University of Tennessee
dc.format.extent276-283
dc.identifierhttp://dx.doi.org/10.1016/j.ijppaw.2014.09.003
dc.identifier.citationInternational Journal for Parasitology: Parasites and Wildlife, v. 3, n. 3, p. 276-283, 2014.
dc.identifier.doi10.1016/j.ijppaw.2014.09.003
dc.identifier.issn2213-2244
dc.identifier.scopus2-s2.0-84908375418
dc.identifier.urihttp://hdl.handle.net/11449/231342
dc.language.isoeng
dc.relation.ispartofInternational Journal for Parasitology: Parasites and Wildlife
dc.sourceScopus
dc.subjectGenetic markers
dc.subjectGenotyping
dc.subjectIsolation
dc.subjectPCR/RFLP
dc.subjectSouth America
dc.subjectT.gondii
dc.titleGenetic characterization of Toxoplasma gondii from Brazilian wildlife revealed abundant new genotypesen
dc.typeArtigo

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