Treadmill Training Increases SIRT-1 and PGC-1 alpha Protein Levels and AMPK Phosphorylation in Quadriceps of Middle-Aged Rats in an Intensity-Dependent Manner

Oliveira, Nara R. C.; Marques, Scherolin O.; Luciano, Thais F.; Pauli, Jose R. [UNESP]; Moura, Leandro P. [UNESP]; Caperuto, Erico; Pieri, Bruno L. S.; Engelmann, Julia; Scaini, Gisele; Streck, Emilio L.; Lira, Fabio S. [UNESP]; Pinho, Ricardo A.; Ropelle, Eduardo R.; Silva, Adelino S. R.; De Souza, Claudio T.

Treadmill Training Increases SIRT-1 and PGC-1 alpha Protein Levels and AMPK Phosphorylation in Quadriceps of Middle-Aged Rats in an Intensity-Dependent Manner

Arquivos

WOS000337829000001.pdf (2.27 MB)

Data

2014-01-01

Autores

Oliveira, Nara R. C.

Marques, Scherolin O.

Editor

Hindawi Publishing Corporation

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

The present study investigated the effects of running at 0.8 or 1.2 km/h on inflammatory proteins (i.e., protein levels of TNF-alpha, IL-1 beta, and NF-kappa B) and metabolic proteins (i.e., protein levels of SIRT-1 and PGC-1 alpha, and AMPK phosphorylation) in quadriceps of rats. Male Wistar rats at 3 (young) and 18 months (middle-aged rats) of age were divided into nonexercised (NE) and exercised at 0.8 or 1.2 km/h. The rats were trained on treadmill, 50 min per day, 5 days per week, during 8 weeks. Forty-eight hours after the last training session, muscles were removed, homogenized, and analyzed using biochemical and western blot techniques. Our results showed that: (a) running at 0.8 km/h decreased the inflammatory proteins and increased the metabolic proteins compared with NE rats; (b) these responses were lower for the inflammatory proteins and higher for the metabolic proteins in young rats compared with middle-aged rats; (c) running at 1.2 km/h decreased the inflammatory proteins and increased the metabolic proteins compared with 0.8 km/h; (d) these responses were similar between young and middle-aged rats when trained at 1.2 km. In summary, the age-related increases in inflammatory proteins, and the age-related declines in metabolic proteins can be reversed and largely improved by treadmill training.