Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post set

dc.contributor.authorDe Rosatto, Camila Maria Peres
dc.contributor.authorSouza, Gabriela Leite de
dc.contributor.authorFerraz, Danilo Cassiano [UNESP]
dc.contributor.authorSilva, Marcelo José Barbosa
dc.contributor.authorTanomaru-Filho, Mario [UNESP]
dc.contributor.authorMoura, Camilla Christian Gomes
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-03-01T19:58:57Z
dc.date.available2023-03-01T19:58:57Z
dc.date.issued2022-01-01
dc.description.abstractSolubility, pH, ion release, cytotoxicity, and osteoclastogenesis inhibition in bone marrow-derived monocyte macrophages (BMMs) were evaluated in EndoSequence BC Sealer (END), Bio-C Sealer (BC), and Sealer Plus BC (SPBC). pH was determined after immersion of the sealers in deionized water (DW) and Minimum Essential Medium Alpha (α-MEM). Solubility was obtained by mass loss. Ion release was measured by using X-ray fluorescence spectroscopy (XRF). Cytotoxicity was evaluated by MTT assay. Inhibition of osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP). Data were analyzed using the t-test, ANOVA and Tukey/Dunnett’s post-hoc tests (α = 0.05). END had the highest pH in DW (p < 0.05), and BC, in α-MEM (p < 0.05). Solubility in DW was the lowest for SPBC (p < 0.005). The highest calcium release was observed for BC in DW at 12 h (p < 0.05), and in α-MEM at 12 and 24 h (p < 0.05). The lowest toxicity was detected for END (p < 0.05). BC had the highest inhibitory effect on osteoclasts (p < 0.05). Overall, the highest solubility and pH values were found in DW. However, the calcium silicate-based sealer showed higher solubility than the ISO standards. Calcium release was the highest for BC. END showed the highest cell viability, and BC, the highest osteoclast inhibition.en
dc.description.affiliationUniversidade Federal de Uberlândia – UFU School of Dentistry Department of Endodontics, MG
dc.description.affiliationUniversidade Estadual Paulista – Unesp School of Dentistry Department of Restorative Dentistry, SP
dc.description.affiliationUniversidade Federal de Uberlândia – UFU Institute of Biomedical Sciences Department of Immunology, MG
dc.description.affiliationUnespUniversidade Estadual Paulista – Unesp School of Dentistry Department of Restorative Dentistry, SP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.identifierhttp://dx.doi.org/10.1590/1807-3107bor-2022.vol36.0065
dc.identifier.citationBrazilian Oral Research, v. 36.
dc.identifier.doi10.1590/1807-3107bor-2022.vol36.0065
dc.identifier.issn1807-3107
dc.identifier.issn1806-8324
dc.identifier.scopus2-s2.0-85129929766
dc.identifier.urihttp://hdl.handle.net/11449/240045
dc.language.isoeng
dc.relation.ispartofBrazilian Oral Research
dc.sourceScopus
dc.subjectEndodontics
dc.subjectMaterials testing
dc.subjectOsteogenesis
dc.subjectRoot canal filling materials
dc.titlePhysicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post seten
dc.typeArtigo
unesp.author.orcid0000-0003-0207-8402[1]
unesp.author.orcid0000-0003-3505-5258[2]
unesp.author.orcid0000-0001-9102-4320[3]
unesp.author.orcid0000-0002-5807-4286[4]
unesp.author.orcid0000-0002-2574-4706[5]
unesp.author.orcid0000-0002-5653-8403[6]

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