Higher expression of Th1/Th2-related cytokines in the intestine of Wistar rats with ligature-induced periodontitis

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dc.contributor.authorde Mello-Neto, João Martins
dc.contributor.authorElangovan, Gayathiri
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorJohnson, Newell Walter
dc.contributor.authorGustafsson, Anders
dc.contributor.authorda Silva Figueredo, Carlos Marcelo
dc.contributor.institutionGriffith University
dc.contributor.institutionJames Cook University
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionKarolinska Institutet
dc.date.accessioned2023-07-29T16:10:40Z
dc.date.available2023-07-29T16:10:40Z
dc.date.issued2023-06-01
dc.description.abstractObjective: This study aimed to investigate the impact of ligature-induced periodontitis (LIP) on histopathological and immunological outcomes in the colon of Wistar rats. Background: It has been repeatedly shown that inflammatory bowel disease (IBD) patients are at higher risk of developing periodontitis and presenting worse oral health than non-IBD patients. However, whether the chronic inflammatory process around teeth contributes to the pathophysiology of IBD needs to be further explored. Materials and Methods: Thirteen Wistar rats were allocated into LIP (n = 7) and controls (n = 6). Half of the colon was processed for histopathological analyses and immunohistochemical (CD45); the other half was homogenized for immunological analyses. Periodontal destruction was confirmed by measuring the distance from the cementum-enamel junction to the mandible's apical position of the mesial interproximal bone. The immunological analyses were performed with the Bio-Plex Th1/Th2 assay. Results: There was a significantly higher interproximal bone loss in LIP compared to controls. The LIP group showed a moderate infiltrate of inflammatory cells, predominantly mononucleated cells in the intestinal tissues. There was significantly higher expression of GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-10, IL-12 (p70), IL-13, and TNF-α in the intestinal tissues of LIP group compared to controls. Conclusion: Ligature-induced periodontitis was associated with an overexpression of Th1/Th2-related cytokines in the colon of Wistar rats.en
dc.description.affiliationSchool of Medicine and Dentistry Griffith University
dc.description.affiliationCollege of Medicine and Dentistry James Cook University
dc.description.affiliationDepartment of Basic Sciences School of Dentistry São Paulo State University-UNESP
dc.description.affiliationDivision of Oral Diseases Department of Dental Medicine Karolinska Institutet
dc.description.affiliationUnespDepartment of Basic Sciences School of Dentistry São Paulo State University-UNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent588-595
dc.identifierhttp://dx.doi.org/10.1111/jre.13121
dc.identifier.citationJournal of Periodontal Research, v. 58, n. 3, p. 588-595, 2023.
dc.identifier.doi10.1111/jre.13121
dc.identifier.issn1600-0765
dc.identifier.issn0022-3484
dc.identifier.scopus2-s2.0-85152034314
dc.identifier.urihttp://hdl.handle.net/11449/249840
dc.language.isoeng
dc.relation.ispartofJournal of Periodontal Research
dc.sourceScopus
dc.subjectcytokines
dc.subjectinflammatory bowel diseases
dc.subjectintestine
dc.subjectperiodontitis
dc.subjectrats
dc.titleHigher expression of Th1/Th2-related cytokines in the intestine of Wistar rats with ligature-induced periodontitisen
dc.typeArtigo

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