Higher expression of Th1/Th2-related cytokines in the intestine of Wistar rats with ligature-induced periodontitis
dc.contributor.author | de Mello-Neto, João Martins | |
dc.contributor.author | Elangovan, Gayathiri | |
dc.contributor.author | Ervolino, Edilson [UNESP] | |
dc.contributor.author | Johnson, Newell Walter | |
dc.contributor.author | Gustafsson, Anders | |
dc.contributor.author | da Silva Figueredo, Carlos Marcelo | |
dc.contributor.institution | Griffith University | |
dc.contributor.institution | James Cook University | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | Karolinska Institutet | |
dc.date.accessioned | 2023-07-29T16:10:40Z | |
dc.date.available | 2023-07-29T16:10:40Z | |
dc.date.issued | 2023-06-01 | |
dc.description.abstract | Objective: This study aimed to investigate the impact of ligature-induced periodontitis (LIP) on histopathological and immunological outcomes in the colon of Wistar rats. Background: It has been repeatedly shown that inflammatory bowel disease (IBD) patients are at higher risk of developing periodontitis and presenting worse oral health than non-IBD patients. However, whether the chronic inflammatory process around teeth contributes to the pathophysiology of IBD needs to be further explored. Materials and Methods: Thirteen Wistar rats were allocated into LIP (n = 7) and controls (n = 6). Half of the colon was processed for histopathological analyses and immunohistochemical (CD45); the other half was homogenized for immunological analyses. Periodontal destruction was confirmed by measuring the distance from the cementum-enamel junction to the mandible's apical position of the mesial interproximal bone. The immunological analyses were performed with the Bio-Plex Th1/Th2 assay. Results: There was a significantly higher interproximal bone loss in LIP compared to controls. The LIP group showed a moderate infiltrate of inflammatory cells, predominantly mononucleated cells in the intestinal tissues. There was significantly higher expression of GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-10, IL-12 (p70), IL-13, and TNF-α in the intestinal tissues of LIP group compared to controls. Conclusion: Ligature-induced periodontitis was associated with an overexpression of Th1/Th2-related cytokines in the colon of Wistar rats. | en |
dc.description.affiliation | School of Medicine and Dentistry Griffith University | |
dc.description.affiliation | College of Medicine and Dentistry James Cook University | |
dc.description.affiliation | Department of Basic Sciences School of Dentistry São Paulo State University-UNESP | |
dc.description.affiliation | Division of Oral Diseases Department of Dental Medicine Karolinska Institutet | |
dc.description.affiliationUnesp | Department of Basic Sciences School of Dentistry São Paulo State University-UNESP | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.format.extent | 588-595 | |
dc.identifier | http://dx.doi.org/10.1111/jre.13121 | |
dc.identifier.citation | Journal of Periodontal Research, v. 58, n. 3, p. 588-595, 2023. | |
dc.identifier.doi | 10.1111/jre.13121 | |
dc.identifier.issn | 1600-0765 | |
dc.identifier.issn | 0022-3484 | |
dc.identifier.scopus | 2-s2.0-85152034314 | |
dc.identifier.uri | http://hdl.handle.net/11449/249840 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Periodontal Research | |
dc.source | Scopus | |
dc.subject | cytokines | |
dc.subject | inflammatory bowel diseases | |
dc.subject | intestine | |
dc.subject | periodontitis | |
dc.subject | rats | |
dc.title | Higher expression of Th1/Th2-related cytokines in the intestine of Wistar rats with ligature-induced periodontitis | en |
dc.type | Artigo |