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A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus

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dc.contributor.authorCardoso, T.C. [UNESP]
dc.contributor.authorMouro-Sousa, R.L. [UNESP]
dc.contributor.authorOliveira, C.
dc.contributor.authorStringhini, G. [UNESP]
dc.contributor.authorAugusto-Pinto, A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMerial Laboratories
dc.date.accessioned2013-09-30T19:43:59Z
dc.date.accessioned2014-05-20T13:42:26Z
dc.date.available2013-09-30T19:43:59Z
dc.date.available2014-05-20T13:42:26Z
dc.date.issued1999-06-01
dc.description.abstractA liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT) were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926) between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in chicken sera, with good sensitivity (88%), specificity (100%) and agreement (95.31%).en
dc.description.affiliationUniversidade Estadual Paulista
dc.description.affiliationMerial Laboratories
dc.description.affiliationUnespUniversidade Estadual Paulista
dc.format.extent747-752
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X1999000600010
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 6, p. 747-752, 1999.
dc.identifier.doi10.1590/S0100-879X1999000600010
dc.identifier.fileS0100-879X1999000600010.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X1999000600010
dc.identifier.urihttp://hdl.handle.net/11449/14760
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.relation.ispartofjcr1.492
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectliquid phase blocking ELISAen
dc.subjecthumoral responseen
dc.subjectinfectious bronchitis virusen
dc.titleA liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virusen
dc.typeArtigo
dspace.entity.typePublication

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