Abordagem metodológica para a valuação do petencial desregulador endócrino de água de beber: estudo com amostras reais

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dc.contributor.advisorCamargo, João Lauro Viana de [UNESP]
dc.contributor.advisorUmbuzeiro, Gisela de Aragão [UNESP]
dc.contributor.authorSolano, Marize de Lourdes Marzo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-05-14T16:53:22Z
dc.date.available2015-05-14T16:53:22Z
dc.date.issued2014-02-10
dc.description.abstractIf contaminated waters are treated by conventional processes (WTP) emerging contaminants are not fully removed and will remain in the drinking water (DW). We investigated the potential for endocrine disruption of two DW samples collected in 2010 and 2012 from a WTP located in São Paulo, Brazil. Water samples were extracted and chemically analyzed for emerging substances and evaluated with an in vitro bioluminescent yeast assay containing estrogen/androgen human receptors (BLYES/BLYAS). In vivo assays were conducted with 21-day old female rats exposed to DW extracts for 03 (uterotrophic assay; OECD) or 20-days (pubertal assay, EPA). The exposure represented a daily ingestion of 2 L, 5 L and 10 L of DW by a 60 kg human being. Caffeine (5.8 - 21 ug/L), estrone (1 ng/L), atrazine (2.2 -11.2 ng/L), carbendazim (0.22 ng/L), azoxistrobina (0.23 ng/L) and tebuconazole (0.19 ng/L) were detected in DW by LC-MS/MS-ESI. There were no increase of the uterus wet weight in the uterotrophic assay, and no alteration of the vaginal opening moment in the pubertal assay. However, there was increased relative blotted uterus weight in animals treated for 3-days with both DW. Levels of LH and FSH presented a significant dose-response increase in the uterotrophic assay developed with the 2010 sample. This happened in association with a significantly increased incidence of vaginal keratinization after the 3-day exposure. Results suggest the DW tested exerted hypothalamic-hypofisis activity alteration in vivo. Additional end-points in standard protocols could be useful to evaluate the presence of endocrine disruptor activity in DWen
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent63 f.
dc.identifier.aleph000829087
dc.identifier.capes33004064056P5
dc.identifier.citationSOLANO, Marize de Lourdes Marzo. Abordagem metodológica para a valuação do petencial desregulador endócrino de água de beber: estudo com amostras reais. 2014. 63 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, 2014.
dc.identifier.filehttp://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-05-2015/000829087.pdf
dc.identifier.urihttp://hdl.handle.net/11449/123331
dc.language.isopor
dc.publisherUniversidade Estadual Paulista (Unesp)
dc.rights.accessRightsAcesso aberto
dc.sourceAleph
dc.subjectÁgua - Qualidadept
dc.subjectÁgua - Poluiçãopt
dc.subjectDesreguladores endócrinospt
dc.subjectContaminantes emergentes na águapt
dc.subjectReação em cadeia de polimerasept
dc.subjectEmerging contaminants in waterpt
dc.subjectWater qualitypt
dc.titleAbordagem metodológica para a valuação do petencial desregulador endócrino de água de beber: estudo com amostras reaispt
dc.title.alternativeA methodological aprroch to evaluate endocrine disrupting activity of drinking water: real samples study in ratsen
dc.typeTese de doutorado
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.graduateProgramPatologia - FMBpt
unesp.knowledgeAreaPatologiapt
unesp.researchAreaPatologia Toxicológicapt

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