Testosterone replacement relieves ligature-induced periodontitis by mitigating inflammation, increasing pro-resolving markers and promoting angiogenesis in rats: A preclinical study

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dc.contributor.authorPelegrin, Álvaro Formoso [UNESP]
dc.contributor.authorde Paiva Gonçalves, Vinícius
dc.contributor.authorCarvalho, Jhonatan de Souza [UNESP]
dc.contributor.authorSpolidorio, Denise Madalena Palomari [UNESP]
dc.contributor.authorSpolidorio, Luís Carlos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionPontifical Catholic University of Minas Gerais
dc.date.accessioned2023-07-29T16:01:30Z
dc.date.available2023-07-29T16:01:30Z
dc.date.issued2023-02-01
dc.description.abstractObjectives: This study aimed to evaluate the inflammatory profile as well as the resolution of inflammation in a ligature-induced periodontal inflammation in rats with depletion and/or supraphysiological testosterone replacement. Design: Sixty male rats (Holtzman) were used in the present study. Study groups were created as following: (1) Sham (no testicle removal); (2) Orchiectomy (OCX), 3) OCX + Testosterone (OCX + T); (4) Sham + Ligature (SH + L); (5) OCX+L; and 6) OCX + T + L. The surgeries were performed on day 1, and testosterone was administered weekly since day 1. On day 15, a cotton ligature was placed around the lower first molars and maintained for 15 days. Morphological changes in periodontal tissues were determined by histopathological analysis. Immunohistochemistry (factor VIII) and immunoenzymatic assay were performed to evaluate angiogenesis process and (pro- and anti-) inflammatory markers, respectively. Results: Ligature promoted a marked inflammatory gingival infiltrate and bone loss (P < 0.05). Supraphysiological testosterone treatment increased the percentage of blood vessels, extracellular matrix and fibroblasts in the presence and absence of periodontal inflammation (P < 0.05). A high dose of testosterone increased factor VIII+ blood vessels and IL-10 expression in inflamed gingival tissue, while PGE2, LXA4 and MPO were reduced as a result of supraphysiological testosterone administration (P < 0.05). Conclusions: These results, in our experimental model, suggest that supraphysiological testosterone treatment stimulated gingival tissue repair during ligature-induced periodontitis, and it seems to be related to an anti-inflammatory and pro-resolutive mechanism resulting by the modulatory effect on PGE2 and IL-10 related to an enhanced angiogenesis.en
dc.description.affiliationDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University – UNESP, 1680 Humaitá St., – Center, SP
dc.description.affiliationDepartment of Dentistry Pontifical Catholic University of Minas Gerais, 500 Dom José Gaspar Avenue, – Coração Eucarístico, MG
dc.description.affiliationDepartment of Physiology and Pathology School of Dentistry São Paulo State University – UNESP, 1680 Humaitá St., – Center, SP
dc.description.affiliationUnespDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University – UNESP, 1680 Humaitá St., – Center, SP
dc.description.affiliationUnespDepartment of Physiology and Pathology School of Dentistry São Paulo State University – UNESP, 1680 Humaitá St., – Center, SP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: #147361/2018-4
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2022.105605
dc.identifier.citationArchives of Oral Biology, v. 146.
dc.identifier.doi10.1016/j.archoralbio.2022.105605
dc.identifier.issn1879-1506
dc.identifier.issn0003-9969
dc.identifier.scopus2-s2.0-85144767303
dc.identifier.urihttp://hdl.handle.net/11449/249504
dc.language.isoeng
dc.relation.ispartofArchives of Oral Biology
dc.sourceScopus
dc.subjectHormone replacement therapy
dc.subjectInflammation
dc.subjectPeriodontal diseases
dc.subjectTestosterone
dc.titleTestosterone replacement relieves ligature-induced periodontitis by mitigating inflammation, increasing pro-resolving markers and promoting angiogenesis in rats: A preclinical studyen
dc.typeArtigo

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