Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA

dc.contributor.authorNascimento, Gustavo Giacomelli
dc.contributor.authorLeite, Amauri Antiquera
dc.contributor.authorLeite, Elza Regina Manzolli
dc.contributor.authorSampaio, José Eduardo Cezar [UNESP]
dc.contributor.authorLeite, Fábio Renato Manzolli
dc.contributor.institutionUniversidade Federal de Pelotas (UFPEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-02-02T12:39:43Z
dc.date.available2015-02-02T12:39:43Z
dc.date.issued2014-04-01
dc.description.abstractAIM: To assess the combined use of tetracycline (TTC) and ethylenediaminetetraacetic acid (EDTA) on clot formation, considering that EDTA may neutralize TTC acidity. METHODS: Planed human tooth roots were treated with saline solution, EDTA, TTC and their combination (EDTA followed by TTC and TTC before EDTA). Fresh human blood was applied on the conditioned surfaces to check clot adhesion and stabilization. A previously calibrated (kappa = 0.93) and blinded examiner scored scanning electron micrographs of the samples. Statistical analyses were performed using one-way ANOVA and Tukey's test. RESULTS: Application of TTC before EDTA presented the best results with the highest number of cells adhered to the root surface (p=0.046). Use of EDTA alone and EDTA before TTC disturbed clot stabilization when compared to control group (p<0.01). CONCLUSIONS: The use of TTC before EDTA seems to be able to keep blood cells viable to establish an organized clot and could be used by clinicians together with the conventional mechanical root scaling and planing.en
dc.description.affiliationUniversidade Federal de Pelotas School of Dentistry Department of Semiology and Clinics
dc.description.affiliationUniversidade Estadual Paulista School of Pharmaceutical Sciences Department of Clinical Analysis
dc.description.affiliationUniversidade Estadual Paulista Araraquara Dental School Department of Diagnosis and Surgery
dc.description.affiliationUnespUniversidade Estadual Paulista School of Pharmaceutical Sciences Department of Clinical Analysis
dc.description.affiliationUnespUniversidade Estadual Paulista Araraquara Dental School Department of Diagnosis and Surgery
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent83-88
dc.identifierhttp://dx.doi.org/10.1590/1677-3225v13n2a01
dc.identifier.citationBrazilian Journal of Oral Sciences. Faculdade de Odontologia de Piracicaba - UNICAMP, v. 13, n. 2, p. 83-88, 2014.
dc.identifier.doi10.1590/1677-3225v13n2a01
dc.identifier.fileS1677-32252014000200083.pdf
dc.identifier.issn1677-3225
dc.identifier.lattes9531839078426485
dc.identifier.scieloS1677-32252014000200083
dc.identifier.urihttp://hdl.handle.net/11449/114631
dc.language.isoeng
dc.publisherFaculdade de Odontologia de Piracicaba - UNICAMP
dc.relation.ispartofBrazilian Journal of Oral Sciences
dc.relation.ispartofsjr0,131
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectethylenediaminetetraacetic aciden
dc.subjectperiodontal regenerationen
dc.subjectscanning electron microscopyen
dc.subjectsmear layeren
dc.titleBlood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTAen
dc.typeArtigo
unesp.author.lattes9531839078426485
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
S1677-32252014000200083.pdf
Tamanho:
229.06 KB
Formato:
Adobe Portable Document Format